Human TNF alpha ELISPOT Ready-SET-Go!®

Also known as: TNF a, TNF alpha, TNFalpha, Tumor necrosis factor alpha

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7398

Cat. No.	Size
88-7398-21	2 x 96 tests
Please inquire about custom configurations or bulk packaging.

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Human PBMC were cultured with PMA (50 ng/ml), ionomycin (500 ng/ml), and LPS (1 ug/ml) for 24 hours...View More

Description

Description: This Human TNF-α ELISPOT Ready-SET-Go! reagent set contains the necessary reagents for performing enzyme linked immunosorbent spot (ELISPOT) assays for high resolution frequency analysis of TNF-α-secreting cells. This ELISPOT reagent set is pre-titrated for optimal spot development.

Details

Reactivity	Human
Components	Capture Antibody. Pre-titrated, Functional Grade (low endotoxin) purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISPOT Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISPOT Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20 and filter with 0.22 uM). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Certificate of Analysis. Lot-specific instructions for dilution of antibodies and enzyme
Reported Applications	ELISPOT

Documentation

TDS Link	Download TDS

References

View More Products

Antigens	Area of Biology	Cell Type
TNF alpha	Apoptosis Cytokines, Chemokines, Growth Factors Innate Immunity	Macrophage & Monocyte Cells NK & NKT Cells T Cells Th1 Cells