Human TNF alpha ELISA Ready-SET-Go!®

Also known as: Tumor Necrosis Factor alpha, TNFa TNF-a, TNF-alpha

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7346

Cat. No. Size
88-7346-22 2 x 96 tests w/plates
88-7346-76 20 x 96 tests w/plates
88-7346-77 20 x 96 tests
88-7346-86 10 x 96 tests w/plates
88-7346-88 10 x 96 tests

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Data for Human TNF alpha ELISA Ready-SET-Go!®.

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  • Data for Human TNF alpha ELISA Ready-SET-Go!®.
Description

Description: This Human TNF-α ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of human TNF-α protein levels from samples including serum, plasma, and supernatants from cell cultures.

Details
Reactivity Human
Sensitivity 4 pg/ml
Standard Curve Range 4 - 500 pg/ml
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

References: Marseille-Tremblay C, Ethier-Chiasson M, Forest JC, Giguère Y, Masse A, Mounier C, Lafond J. Impact of maternal circulating cholesterol and gestational diabetes mellitus on lipid metabolism in human term placenta. Mol Reprod Dev. 2008 Jun;75(6):1054-62. (RSG ELISA kit, tissue homogenate, PubMed)

Tversky JR, Le TV, Bieneman AP, Chichester KL, Hamilton RG, Schroeder JT. Human blood dendritic cells from allergic subjects have impaired capacity to produce interferon-alpha via Toll-like receptor 9. Clin Exp Allergy. 2008 May;38(5):781-8. (RSG ELISA kit, TC supernatant, PubMed)

Ahuja J, Lepoutre V, Wigdahl B, Khan ZK, Jain P. Induction of pro-inflammatory cytokines by human T-cell leukemia virus type-1 Tax protein as determined by multiplexed cytokine protein array analyses of human dendritic cells. Biomed Pharmacother. 2007 May;61(4):201-8. (RSG ELISA kit, TC supernatant, PubMed)

Utz JP, Limper AH, Kalra S, Specks U, Scott JP, Vuk-Pavlovic Z, Schroeder DR. Etanercept for the treatment of stage II and III progressive pulmonary sarcoidosis. Chest. 2003 Jul;124(1):177-85. (RSG ELISA kit, TC supernatant, serum, and lavage fluid, PubMed)