Anti-Human TNF alpha Alexa Fluor® 488

Also known as: Tumor Necrosis Factor alpha

Clone: MAb11

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-7349

Cat. No.	Size
53-7349-41	25 tests
53-7349-42	100 tests
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Intracellular staining of 5-hour PMA/Ionomycin stimulated (in the presence of brefeldin A) normal...View More

Description

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Details

Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
56-7349	Anti-Human TNF alpha Alexa Fluor® 700	633 - 647 nm	723 nm	FC	RUO
12-7349	Anti-Human TNF alpha PE	488 - 561 nm	575 nm	FC	RUO
14-7349	Anti-Human TNF alpha Purified			FC	RUO
11-7349	Anti-Human TNF alpha FITC	488 nm	518 nm	FC	RUO
48-7349	Anti-Human TNF alpha eFluor® 450	405 nm	450 nm	FC	RUO
13-7349	Anti-Human TNF alpha Biotin			ELISA	RUO
45-7349	Anti-Human TNF alpha PerCP-Cyanine5.5	488 nm		FC	RUO
17-7349	Anti-Human TNF alpha APC	633 - 647 nm	660 nm	FC	RUO
25-7349	Anti-Human TNF alpha PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
14-7348	Anti-Human TNF alpha Purified			ELISA	RUO
16-7348	Anti-Human TNF alpha Functional Grade Purified			ELISA, FA	RUO
17-0048	Anti-Human CD4 APC	633 - 647 nm	660 nm	FC	RUO
53-4714	Mouse IgG1 K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO
88-8823	Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) (previously named IC Fixation & Permeabilization Buffer)			FC	GPR

References

References: Andersen H, Rossio JL, Coalter V, Poore B, Martin MP, Carrington M, Lifson JD. Characterization of rhesus macaque natural killer activity against a rhesus-derived target cell line at the single-cell level. Cell Immunol. 2004 Sep-Oct;231(1-2):85-95 (Mab11, crossreactivity to rhesus)

Danis VA, Franic GM, Rathjen DA, Brooks PM. Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes. Clin Exp Immunol. 1991 Jul;85(1):143-50.

Rathjen DA, Cowan K, Furphy LJ, Aston R. Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors. Mol Immunol. 1991 Jan-Feb;28(1-2):79-86.

View More Products

Antigens	Area of Biology	Cell Type	Formats
TNF alpha	Apoptosis Cytokines, Chemokines, Growth Factors Innate Immunity	Macrophage & Monocyte Cells NK & NKT Cells T Cells Th1 Cells	Alexa Fluor 488