Human TIMP-1 FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human TIMP-1 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect TIMP-1 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population B5.

TThe tissue inhibitions of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases, thus maintaining balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors.

TIMP-1 has been shown to inhibit tumor-induced angiogenesis. An imbalance of MMP/TIMP regulation has been implicated in several inflammatory diseases of the nervous system. In the pathogenesis of vascular diseases TIMP-1 seems to be down regulated.

References: Wojtowicz-Praga,S.M.; Dickson,R.B.; Hawkins,M.J.. Matrix metalloproteinase inhibitors. Invest New Drugs 1997;15:61-75. (Link)

Hornebeck,W.. Down-regulation of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival. Pathol.Biol.(Paris) 2003;51:569-573. (Link)

Yan,L.; Moses,M.A.. A case of tumor betrayal: biphasic effects of TIMP-1 on Burkitt's lymphoma. Am.J.Pathol. 2001;158:1185-1190. (Link)

Shin,W.S.; Szuba,A.; Rockson,S.G.. The role of chemokines in human cardiovascular pathology: enhanced biological insights. Atherosclerosis 2002;160:91-102. (Link)

Nagase,H.; Meng,Q.; Malinovskii,V.; Huang,W.; Chung,L.; Bode,W.; Maskos,K.; Brew,K.. Engineering of selective TIMPs. Ann.N.Y.Acad.Sci. 1999;878:1-11. (Link)

Thorgeirsson,U.P.; Lindsay,C.K.; Cottam,D.W.; Gomez,D.E.. Tumor invasion, proteolysis, and angiogenesis. J.Neurooncol. 1994;18:89-103. (Link)

Gardner,J.; Ghorpade,A.. Tissue inhibitor of metalloproteinase (TIMP)-1: the TIMPed balance of matrix metalloproteinases in the central nervous system. J.Neurosci.Res. 2003;74:801-806. (Link)

Arthur,M.J.; Iredale,J.P.. Hepatic lipocytes, TIMP-1 and liver fibrosis. J.R.Coll.Physicians Lond 1994;28:200-208. (Link)

Nagase,H.; Brew,K.. Designing TIMP (tissue inhibitor of metalloproteinases) variants that are selective metalloproteinase inhibitors. Biochem.Soc.Symp. 2003;201-212. (Link)