Human Th1/Th2 ELISA Ready-SET-Go!® (Discontinued. Please refer to IL-2, IL-4, IL-10, & IFN gamma ELISAs)

Also known as: IFNg, IL-10, IL-2, IL-4

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7799

Cat. No.	Size
88-7799-44	Panel
Please inquire about custom configurations or bulk packaging.

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Description

Description: The eBioscience Human Th1/Th2 ELISA Ready-SET-Go! contains 4 pairs of antibodies that can be used to discriminate Th1/Th2 subsets based on their expression of IFN-γ, IL-2, IL-4 and IL-10. This reagent panel contains all of the necessary antibody reagents, standards, avidin-HRP, TMB substrate solution, coating buffers and diluents for performing two 96-well ELISA plate assays for each of these four cytokines. This ELISA reagent set is specifically engineered for accurate and precise measurement of cytokine protein levels from samples including serum, plasma, and supernatants from cell cultures.

Details

Reactivity	Human
Sensitivity	IFN-gamma: 4 pg/ml IL-2: 4 pg/ml IL-4: 2 pg/ml IL-10: 2 pg/ml
Standard Curve Range	IFN-gamma: 4 - 500 pg/ml IL-2: 4 - 500 pg/ml IL-4: 2 - 200 pg/ml IL-10: 2 - 300 pg/ml
Components	Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples ELISA/ELISPOT Coating Buffer Powder. Reconstitute to 1L with dH20 and filter (0.22 uM). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards 96 Well Plate. Corning Costar 9018 (included)
Reported Applications	ELISA

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

References

References: Baba M, Yong Ma B, Nonaka M, Matsuishi Y, Hirano M, Nakamura N, Kawasaki N, Kawasaki N, Kawasaki T. Glycosylation-dependent interaction of Jacalin with CD45 induces T lymphocyte activation and Th1/Th2 cytokine secretion. J Leukoc Biol. 2007 Apr;81(4):1002-11. (RSG ELISA kit, TC supernatant, PubMed)

Atègbo JM, Grissa O, Yessoufou A, Hichami A, Dramane KL, Moutairou K, Miled A, Grissa A, Jerbi M, Tabka Z, Khan NA. Modulation of adipokines and cytokines in gestational diabetes and macrosomia. J Clin Endocrinol Metab. 2006 Oct;91(10):4137-43. (RSG ELISA kit, serum, PubMed)

View More Products

Antigens	Area of Biology	Cell Type
IFN gamma IL-10 IL-2 IL-4	Cytokines, Chemokines, Growth Factors	Macrophage & Monocyte Cells NK & NKT Cells T Cells Treg Cells Th1 Cells Th2 Cells