TCR alpha/beta PE

Description: The WT31 monoclonal antibody recognizes the human alpha/beta T cell receptor (TCR) complexed with the CD3 epsilon chain. Composed of a 44-kDa alpha and a 37-kDa beta chain, TCR specificity is typically determined by V alpha, J alpha, V beta, D beta, and J beta gene rearrangement. The TCR is often associated with the CD3 complex, which is composed of at least five polypeptides. The TCR/CD3 complex recognizes and binds to major histocompatibility complex-bound antigen presented by antigen presenting cells such as B cells, dendritic cells, and macrophages. These events can lead to T cell activation, resulting in cellular signaling and cytokine production.

Some antibody clones specific for CD3 partially inhibit binding of WT31.

References: Salmerón A, Sánchez-Madrid F, Ursa MA, Fresno M, Alarcón B. A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. J Immunol. 1991 Nov 1;147(9):3047-52.

Alarcon B, De Vries J, Pettey C, Boylston A, Yssel H, Terhorst C, Spits H. The T-cell receptor gamma chain-CD3 complex: implication in the cytotoxic activity of a CD3+ CD4- CD8- human natural killer clone. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3861-5.

Spits H, Borst J, Tax W, Capel PJ, Terhorst C, de Vries JE. Characteristics of a monoclonal antibody (WT-31) that recognizes a common epitope on the human T cell receptor for antigen. J Immunol. 1985 Sep;135(3):1922-8.