Human t-PA Platinum ELISA

Description: The human t-PA ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human t-PA. The human t-PA ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Tissue-type plasminogen activator (t-PA) is a serine protease which occurs in blood plasma, serum, other body fluids, tissues and conditioned media of certain cultured cells. It can convert the inactive proenzyme plasminogen to the active protease plasmin. Plasmin can degrade fibrin, the matrix of a blood clot in a process known as fibrinolysis, leading to dissolution of the clot. Furthermore plasminogen activation is implicated in metastatic spread of malignant cells and in tissue remodelling.

Elevated levels of t-PA in serum were shown to occur in relation to retinopathy in type 1 diabetes mellitus. The major field of clinical interest is the field of diseases of the heart. t-PA plasma levels have been shown to be altered with the presence of transplant coronary artery disease in cardiac transplant recipients. A correlation between low serum levels of t-PA activity and thrombotic tendency has been described.

Citations: Demyanets,Svitlana; Kaun,Christoph; Rychli,Kathrin; Rega,Gersina; Pfaffenberger,Stefan; Afonyushkin,Taras; Bochkov,Valery N.; Maurer,Gerald; Huber,Kurt; Wojta,Johann. The inflammatory cytokine oncostatin M induces PAI-1 in human vascular smooth muscle cells in vitro via PI3 kinase and ERK 1/2 dependent pathways. Heart and Circulatory Physiology 2007;01366(Link)

Polubinska,Alicja; Breborowicz,Andrzej; Staniszewski,Ryszard; Oreopoulos,Dimitrios G.. Normal saline induces oxidative stress in peritoneal mesothelial cells. Journal of Pediatric Surgery 2008;43:1821-1826. (Link)

References: Mehta,J.; Mehta,P.; Lawson,D.; Saldeen,T.. Plasma tissue plasminogen activator inhibitor levels in coronary artery disease: correlation with age and serum triglyceride concentrations. J.Am.Coll.Cardiol. 1987;9:263-268. (Link)

Okabe,K.; Kato,I.; Sato,S.; Kashiwabara,T.; Furuta,S.; Sato,H.; Tanikawa,K.; Kumashiro,R.; Kanayama,M.. Clinical evaluation of tissue plasminogen activator (t-PA) levels in patients with liver diseases. Gastroenterol.Jpn. 1992;27:61-68. (Link)

Li,Y.; Chen,H.; Tan,J.; Wang,X.; Liang,H.; Sun,X.. Impaired release of tissue plasminogen activator from the endothelium in Graves' disease - indicator of endothelial dysfunction and reduced fibrinolytic capacity. Eur.J.Clin.Invest 1998;28:1050-1054. (Link)

Olofsson,B.O.; Dahlen,G.; Nilsson,T.K.. Evidence for increased levels of plasminogen activator inhibitor and tissue plasminogen activator in plasma of patients with angiographically verified coronary artery disease. Eur.Heart J. 1989;10:77-82. (Link)

Hara,M.; Ito,K.; Nawata,T.; Tsunematsu,Y.; Shimoyama,N.; Maeda,T.; Sato,Y.; Saikawa,T.; Sakata,T.. Plasma plasminogen activator inhibitor-1, tissue plasminogen activator and serum lipoprotein(a) after reperfusion therapy in acute myocardial infarction: comparison between sequential and direct percutaneous transluminal coronary angioplasty. Cardiology 1995;86:407-410. (Link)

Skrha,J.; Hodinar,A.; Kvasnicka,J.; Stibor,V.; Sperl,M.; Stolba,P.; Hilgertova,J.. Early changes of serum N-acetyl-beta-glucosaminidase, tissue plasminogen activator and erythrocyte superoxide dismutase in relation to retinopathy in type 1 diabetes mellitus. Clin.Chim.Acta 1994;229:5-14. (Link)

Smith,F.B.; Lee,A.J.; Rumley,A.; Fowkes,F.G.; Lowe,G.D.. Tissue-plasminogen activator, plasminogen activator inhibitor and risk of peripheral arterial disease. Atherosclerosis 1995;115:35-43. (Link)

Warshofsky,M.K.; Wasserman,H.S.; Wang,W.; Teng,P.; Sciacca,R.; Apfelbaum,M.; Schwartz,A.; Michler,R.E.; Mancini,D.M.; Cannon,P.J.; Rabbani,L.E.. Plasma levels of tissue plasminogen activator and plasminogen activator inhibitor-1 are correlated with the presence of transplant coronary artery disease in cardiac transplant recipients. Am.J.Cardiol. 1997;80:145-149. (Link)

Hamsten,A.; Wiman,B.; de,Faire U.; Blomback,M.. Increased plasma levels of a rapid inhibitor of tissue plasminogen activator in young survivors of myocardial infarction. N.Engl.J.Med. 1985;313:1557-1563. (Link)