Human sVE-cadherin Platinum ELISA

Description: The human VE-cadherin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VE-cadherin. The human VE-cadherin ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Cadherin-5, though member of the family of cadherins has been shown to be functionally as well as structurally distinct from classical cadherins (e.g. E-, N-, P-cadherins). Through its function and location cadherin-5 has been named VE-cadherin. Its a protein of a relative molecular mass of about 130 kDa.

The vascular endothelial cadherin (VE-cadherin) gene encodes a Ca2+-dependent cell adhesion molecule required for the organization of interendothelial junctions. VE-cadherin mediates homophilic, calcium-dependent aggregation and cell-to-cell adhesion. In addition, it decreases intercellular permeability to high-molecular weight molecules and reduces cell migration rate across a wounded area. VE-cadherin is a transmembrane protein, the intracellular domain has been shown to interact with cytoplasmic proteins called catenins that transmit the adhesion signal upon this activation. So the VE-cadherin extracellular domain is enough for early steps of cell adhesion and recognition. However, interaction of VE-cadherin the cytoskeleton, mediated through the cytoplasmatic domain, is necessary to provide strength and cohesion to the junction.

The initiation of endothelial apoptosis correlates with cleavage and disassembly of components of adherens junctions. The extracellular portion of these junctions is altered during apoptosis because VE-cadherin dramatically decreases on the surface of cells. An extracellular fragment of VE-cadherin can be detected. This shedding of VE-cadherin can be blocked by an inhibitor of metalloproteinases. It may be part of a concerted mechanism to disrupt structural and signaling properties of adherens junctions and may actively interrupt extracellular signals required for endothelial cell survival.

Citations: Schulz,Beate; Pruessmeyer,Jessica; Maretzky,Thorsten; Ludwig,Andreas; Blobel,Carl P.; Saftig,Paul; Reiss,Karina. ADAM10 Regulates Endothelial Permeability and T-Cell Transmigration by Proteolysis of Vascular Endothelial Cadherin. Circulation Research 2008;102:1192-1201. (Link)

WROBEL,T.; MAZUR,G.; WOLOWIEC,D.; JAZWIEC,B.; SOWINSKA,E.; KULICZKOWSKI,K.. sVE-cadherin and sCD146 serum levels in patients with multiple myeloma. Clinical and Laboratory Haematology 2006;28:36-39. (Link)

Villasante,A.; Pacheco,A.; Pau,E.; Ruiz,A.; Pellicer,A.; Garcia-Velasco,J.A.. Soluble vascular endothelial-cadherin levels correlate with clinical and biological aspects of severe ovarian hyperstimulation syndrome. Human Reproduction 2008;23:662-667. (Link)

Villasante,A.; Pacheco,A.; Ruiz,A.; Pellicer,A.; Garcia-Velasco,J.A.. Vascular endothelial cadherin regulates vascular permeability: Implications for ovarian hyperstimulation syndrome. Journal of Clinical Endocrinology & Metabolism 2006;92:jc(Link)

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