Anti-Human SSEA-4 eFluor® 570

Also known as: stage-specific embryonic antigen-4

Clone: eBioMC-813-70 (MC-813-70)

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 41-8843

Cat. No. Size
41-8843-80 25 ug
41-8843-82 100 ug
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Data for Anti-Human SSEA-4 eFluor® 570.

Immunocytochemistry on fixed NTERA-2 cells using 10 ug/mL Anti-Human SSEA-4 eFluor® 570. Nuclei are...View More

  • Data for Anti-Human SSEA-4 eFluor® 570.
Description

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

Details
Host Mouse
Isotype IgG3
Reactivity Canine, Chicken, Human, Mouse, Rabbit
Conjugate eFluor 570
Emit 570 nm
Excite 555 nm
Reported Applications Immunocytochemistry
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
14-8843 Anti-Human SSEA-4 Purified FC, IHC RUO
53-8843 Anti-Human SSEA-4 Alexa Fluor® 488 488 nm 519 nm FC RUO
46-8843 Anti-Human SSEA-4 PerCP-eFluor® 710 488 nm 710 nm FC RUO
12-8843 Anti-Human SSEA-4 PE 488 - 561 nm FC RUO
50-8843 Anti-Human SSEA-4 eFluor® 660 633 - 647 nm 668 nm FC, ICC RUO
13-8843 Anti-Human SSEA-4 Biotin FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-4953 IHC /ICC Blocking Buffer - Low Protein ICC, IHC GPR
00-4954 20X TBS Wash Buffer for IHC/ICC ICC, IHC GPR
00-4958 Fluoromount-G™ ICC, IHC GPR
References

References: Williams SA, Stanley P. Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or Le(Y) in the preimplantation mouse embryo. Glycoconj J. 2009 Apr;26(3):335-47. (mouse reactivity)

Müller T, Fleischmann G, Eildermann K, Mätz-Rensing K, Horn PA, Sasaki E, Behr R A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics. Hum Reprod. 2009 Feb 27. (marmoset reactvity)

Shiue YL, Tailiu JJ, Liou JF, Lu HT, Tai C, Shiau JW, Chen LR. Establishment of the long-term in vitro culture system for chicken primordial germ cells. Reprod Domest Anim. 2009 Feb;44(1):55-61 (chicken reactivity)

Wilcox JT, Semple E, Gartley C, Brisson BA, Perrault SD, Villagómez DA, Tayade C, Becker S, Lanza R, Betts DH. Characterization of canine embryonic stem cell lines derived from different niche microenvironments. Stem Cells Dev. 2009 Jan 9. (canine reactivity)

Müller T, Eildermann K, Dhir R, Schlatt S, Behr R. Glycan stem-cell markers are specifically expressed by spermatogonia in the adult non-human primate testis. Hum Reprod. 2008 Oct;23(10):2292-8 (Callithrix jacchus, Macaca mulatta and Macaca silenus crossreactivity)

Gang EJ, Bosnakovski D, Figueiredo CA, Visser JW, Perlingeiro RC. SSEA-4 identifies mesenchymal stem cells from bone marrow. Blood. 2007 Feb 15;109(4):1743-51.

Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 1983;2(12):2355-61.

Krupnick JG, Damjanov I, Damjanov A, Zhu ZM, Fenderson BA. Globo-series carbohydrate antigens are expressed in different forms on human and murine teratocarcinoma-derived cells. Int J Cancer. 1994 Dec 1;59(5):692-8.


View More Products

AntigensCell TypeFormats