Anti-Human SSEA-4 Alexa Fluor® 488

Also known as: stage-specific embryonic antigen-4

Clone: eBioMC-813-70 (MC-813-70)

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-8843

Cat. No. Size
53-8843-41 25 tests
53-8843-42 100 tests
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Data for Anti-Human SSEA-4 Alexa Fluor® 488.

Staining of 2102Ep cell line with Mouse IgG3 Isotype Control FITC (cat. 11-4742) (open histogram)...View More

  • Data for Anti-Human SSEA-4 Alexa Fluor® 488.
Description

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

Details
Host Mouse
Isotype IgG3
Reactivity Canine, Chicken, Human, Mouse, Rabbit
Conjugate Alexa Fluor 488
Laser Blue Laser
Emit 519 nm
Excite 488 nm
Legal Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
50-8843 Anti-Human SSEA-4 eFluor® 660 633 - 647 nm 668 nm FC, ICC RUO
13-8843 Anti-Human SSEA-4 Biotin FC RUO
12-8843 Anti-Human SSEA-4 PE 488 - 561 nm FC RUO
41-8843 Anti-Human SSEA-4 eFluor® 570 555 nm 570 nm ICC RUO
14-8843 Anti-Human SSEA-4 Purified FC, IHC RUO
46-8843 Anti-Human SSEA-4 PerCP-eFluor® 710 488 nm 710 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-4742 Mouse IgG3 Isotype Control FITC 488 nm FC RUO
12-8813 Anti-Human/Mouse SSEA-1 PE 488 - 561 nm 575 nm FC RUO
12-8833 Anti-Human/Mouse SSEA-3 PE 488 - 561 nm 575 nm FC RUO
12-8863 Anti-Human TRA-1-60 (Podocalyxin) PE 488 - 561 nm 575 nm FC RUO
12-8883 Anti-Human TRA-1-81 (Podocalyxin) PE 488 - 561 nm 575 nm FC RUO
References

References: Williams SA, Stanley P. Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or Le(Y) in the preimplantation mouse embryo. Glycoconj J. 2009 Apr;26(3):335-47. (mouse reactivity)

Müller T, Fleischmann G, Eildermann K, Mätz-Rensing K, Horn PA, Sasaki E, Behr R A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics. Hum Reprod. 2009 Feb 27. (marmoset reactvity)

Shiue YL, Tailiu JJ, Liou JF, Lu HT, Tai C, Shiau JW, Chen LR. Establishment of the long-term in vitro culture system for chicken primordial germ cells. Reprod Domest Anim. 2009 Feb;44(1):55-61 (chicken reactivity)

Wilcox JT, Semple E, Gartley C, Brisson BA, Perrault SD, Villagómez DA, Tayade C, Becker S, Lanza R, Betts DH. Characterization of canine embryonic stem cell lines derived from different niche microenvironments. Stem Cells Dev. 2009 Jan 9. (canine reactivity)

Müller T, Eildermann K, Dhir R, Schlatt S, Behr R. Glycan stem-cell markers are specifically expressed by spermatogonia in the adult non-human primate testis. Hum Reprod. 2008 Oct;23(10):2292-8 (Callithrix jacchus, Macaca mulatta and Macaca silenus crossreactivity)

Gang EJ, Bosnakovski D, Figueiredo CA, Visser JW, Perlingeiro RC. SSEA-4 identifies mesenchymal stem cells from bone marrow. Blood. 2007 Feb 15;109(4):1743-51.

Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 1983;2(12):2355-61.

Krupnick JG, Damjanov I, Damjanov A, Zhu ZM, Fenderson BA. Globo-series carbohydrate antigens are expressed in different forms on human and murine teratocarcinoma-derived cells. Int J Cancer. 1994 Dec 1;59(5):692-8.


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