Human sIL-6R Platinum ELISA

Description: The human sIL-6R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sIL-6R. The human sIL-6R ELISA is for research use only. Not for diagnostic or therapeutic procedures.

IL-6 is a multifunctional cytokine involved in the regulation of the immune response, hematopoiesis and acute phase response. It has been recognized to be a member of the alpha-helical cytokine family. IL-6 exerts its action via a cell surface receptor which consists of two subunits, an 80 kDa ligand binding subunit (gp80) of 468 amino acids and a 130 kDa signal transducing protein (gp130) of 896 amino acid residues. Characterisation of the extracellular portion of the 80 kDa IL-6 receptor revealed the existence of a single immunoglobulin-like domain in the NH2-terminal of the extracellular region, which does not contribute to ligand binding. The remainder of the extracellular domain however is essential for low affinity ligand binding, which consecutively triggers the association of the receptor and gp130 thus forming a high affinity binding site for IL-6.

A soluble form of the human gp80 protein has been detected in serum and urine samples. This 55 kDa protein representing the extracellular portion of gp80 is generated by shedding, a process that seems to be controlled by protein kinase C. It is still functional, indicating that soluble gp80 plays a biological role in promoting IL-6 activity. So far, the soluble IL-6 receptor is unique in acting as an agonist together with its ligand.

Citations: Annique,S.; Dorien,T.; Richel,L.; Gunter,K.; Joris,D.; Crijns Harry,J.; Gert,G.; Frank,S.; Michael,M.; Adriaan,H.. Inflammatory markers in depressed post-myocardial infarction patients. Journal of Psychiatric Research 2005;39:137-144. (Link)

Genesca,Joan; Marti,Ramon; Gonzalez,Antonio; Torregrosa,Mireia; Segura,Rosa. Soluble Interleukin-6 Receptor Levels in Liver Cirrhosis. The American Journal of Gastroenterology 1999;94:3074-3075. (Link)

References: Yamasaki,K.; Taga,T.; Hirata,Y.; Yawata,H.; Kawanishi,Y.; Seed,B.; Taniguchi,T.; Hirano,T.; Kishimoto,T.. Cloning and expression of the human interleukin-6 (BSF-2/IFN beta 2) receptor. Science 1988;241:825-828. (Link)

Van,Snick J.. Interleukin-6: an overview. Annu.Rev.Immunol. 1990;8:253-278. (Link)

Fernandez-Botran,R.. Soluble cytokine receptors: their role in immunoregulation. FASEB J. 1991;5:2567-2574. (Link)

Jucker,M.; Abts,H.; Li,W.; Schindler,R.; Merz,H.; Gunther,A.; von,Kalle C.; Schaadt,M.; Diamantstein,T.; Feller,A.C.; .. Expression of interleukin-6 and interleukin-6 receptor in Hodgkin's disease. Blood 1991;77:2413-2418. (Link)

Taga,T.; Hibi,M.; Hirata,Y.; Yamasaki,K.; Yasukawa,K.; Matsuda,T.; Hirano,T.; Kishimoto,T.. Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130. Cell 1989;58:573-581. (Link)

Novick,D.; Engelmann,H.; Wallach,D.; Rubinstein,M.. Soluble cytokine receptors are present in normal human urine. J.Exp.Med. 1989;170:1409-1414. (Link)

Mullberg,J.; Schooltink,H.; Stoyan,T.; Heinrich,P.C.; Rose-John,S.. Protein kinase C activity is rate limiting for shedding of the interleukin-6 receptor. Biochem.Biophys.Res.Commun. 1992;189:794-800. (Link)

Gaillard,J.P.; Bataille,R.; Brailly,H.; Zuber,C.; Yasukawa,K.; Attal,M.; Maruo,N.; Taga,T.; Kishimoto,T.; Klein,B.. Increased and highly stable levels of functional soluble interleukin-6 receptor in sera of patients with monoclonal gammopathy. Eur.J.Immunol. 1993;23:820-824. (Link)

Kishimoto,T.; Akira,S.; Taga,T.. Interleukin-6 and its receptor: a paradigm for cytokines. Science 1992;258:593-597. (Link)

Hibi,M.; Murakami,M.; Saito,M.; Hirano,T.; Taga,T.; Kishimoto,T.. Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell 1990;63:1149-1157. (Link)

Mullberg,J.; Schooltink,H.; Stoyan,T.; Gunther,M.; Graeve,L.; Buse,G.; Mackiewicz,A.; Heinrich,P.C.; Rose-John,S.. The soluble interleukin-6 receptor is generated by shedding. Eur.J.Immunol. 1993;23:473-480. (Link)

Heinrich,P.C.; Castell,J.V.; Andus,T.. Interleukin-6 and the acute phase response. Biochem.J. 1990;265:621-636. (Link)

Brown,R.D.; Gorenc,B.; Gibson,J.; Joshua,D.. Interleukin-6 receptor expression and saturation on the bone marrow cells of patients with multiple myeloma. Leukemia 1993;7:221-225. (Link)

Lust,J.A.; Donovan,K.A.; Kline,M.P.; Greipp,P.R.; Kyle,R.A.; Maihle,N.J.. Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor. Cytokine 1992;4:96-100. (Link)

Honda,M.; Yamamoto,S.; Cheng,M.; Yasukawa,K.; Suzuki,H.; Saito,T.; Osugi,Y.; Tokunaga,T.; Kishimoto,T.. Human soluble IL-6 receptor: its detection and enhanced release by HIV infection. J.Immunol. 1992;148:2175-2180. (Link)