Description: This Human sIL-2R FlowCytomix Simplex Kit is designed for the measurement of human sIL-2R in a bead based immunoassay analyzed on a flow cytometer.
eBioscience bead-based immunoassays follow the same principle as a sandwich immunoassay.
Basic Kits include essential core reagents and are required to successfully perform assays using combinations of Simplex Kits.
IL-2 has been described as a factor that promotes the growth and proliferation of human T-cells thus occupying a pivotal role in the generation of the immune response. This proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific cell surface receptor following T lymphocyte activation. The receptor for IL-2 is composed of at least three distinct polypeptide subunits , the IL-2R, IL-2R, and the IL-2R chains giving rise to a 55-65 kD membrane bound protein.
Evidence has accumulated that suggests a critical role of IL-2R in IL-2 signal transduction. IL-2R coupled tyrosine kinases are believed to play a crucial role in that signal transduction.
In addition to the de-novo expression of IL-2R by activated peripheral blood T-cells, a released and fully soluble form of IL-2R (sIL-2R) has been detected. It was shown that sIL-2R is present in vivo, at low levels in the sera of healthy persons and at markedly elevated levels in various pathological conditions like neoplastic disorders.
Soluble IL-2R levels are significantly elevated in the sera of patients with adult T-cell leukemia, hairy cell leukemia, lymphocytic leukemia, Hodgkin's and Non-Hodgkin's lymphoma, liver, breast and lung cancer.