Welcome to eBioscience

  • Home /
  • Human sICAM-1 Platinum ELISA (extra sensitive)

Human sICAM-1 Platinum ELISA (extra sensitive)

Also known as: Intercellular adhesion molecule 1, CD54 antigen

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS241*

Cat. No. Size
BMS241 96 tests
BMS241TEN 10 x 96 tests
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Data for Human sICAM-1 Platinum ELISA (extra sensitive).

Click on image to view more information.

  • Data for Human sICAM-1 Platinum ELISA (extra sensitive).
Description

Description: The human sICAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sICAM-1. The human sICAM-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-betacomplex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors.

ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B cells and dendritic cells in tonsils, lymph nodes and Peyer's patches.

ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.

sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation.

Due to unique Antibody properties, this ELISA has increased sensitivity without further Amplification steps required.

Details
Reactivity Human
Sample Volume 100 uL (1:100 prediluted)
Suitable Sample Types cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid, spontaneous urine, bile
Sensitivity 0.33 ng/mL
Standard Curve Range 0.63 - 10.0 ng/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-1
HRP-Conjugate anti-human sICAM-1 monoclonal antibody
Human sICAM-1 Standard, 10 ng/ml
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

Citations: Beeh,Kai M.; Beier,Jutta; Kornmann,Oliver; Mander,Annette; Buhl,Roland. Long-term Repeatability of Induced Sputum Cells and Inflammatory Markers in Stable, Moderately Severe COPD. Chest 2003;123:778-783. (Link)

Triolo; Triolo; Accardo,Palumbo; Carbone; Ferrante; Casiglia; Giardina. IgG anti-endothelial cell antibodies (AECA) in type I diabetes mellitus; induction of adhesion molecule expression in cultured endothelial cells. Clinical and Experimental Immunology 1998;111:491-496. (Link)

Piconi,L.; Quagliaro,L.; Da Ros,R.; Assaloni,R.; Giugliano,D.; Esposito,K.; Szabo,C.; Ceriello,A.. Intermittent high glucose enhances ICAM-1, VCAM-1, E-selectin and interleukin-6 expression in human umbilical endothelial cells in culture: the role of poly(ADP-ribose) polymerase. Journal of Thrombosis and Haemostasis 2004;2:1453-1459. (Link)

Chan,Loretta; Leung,Joseph; Tsang,Anita; Tang,Sydney; Lai,Kar. Activation of tubular epithelial cells by mesangial-derived TNF-[alpha]: Glomerulotubular communication in IgA nephropathy. Kidney Int 2005;67:602-612. (Link)

Chan J.C.N.,J C K Cheung,C D A Stehouwer,J J Emeis,P C Y Tong,G T C Ko and J S Yudkin. The central roles of obesity-associated dyslipidaemia, endothelial activation and cytokines in the Metabolic Syndromean analysis by structural equation modelling. International Journal of Obesity 2002;26:994-1008. (Link)

Zabad,R.K.; Metz,L.M.; Todoruk,T.R.; Zhang,Y.; Mitchell,J.R.; Yeung,M.; Patry,D.G.; Bell,R.B.; Yong,V.W.. The clinical response to minocycline in multiple sclerosis is accompanied by beneficial immune changes: a pilot study. Multiple Sclerosis 2007;13:517-526. (Link)

Reindl,M.; Lutterotti,A.; Ingram,J.; Schanda,K.; Gassner,C.; Deisenhammer,F.; Berger,T.; Lorenz,E.. Mutations in the gene for toll-like receptor 4 and multiple sclerosis. Tissue Antigens 2003;61:85-88. (Link)

Hammett,Christopher J.K.; Prapavessis,Harry; Baldi,J.Chris; Varo,Nerea; Schoenbeck,Uwe; Ameratunga,Rohan; French,John K.; White,Harvey D.; Stewart,Ralph A.H.. Effects of exercise training on 5 inflammatory markers associated with cardiovascular risk. American Heart Journal 2006;151:(Link)

Stefanska,Anna; Sypniewskay,Grazyna; Senterkiewicz,Lilla. Inflammatory Markers and Cardiovascular Risk in Healthy Polish Women across the Menopausal Transition. Clinical Chemistry 2005;51:1893-1895. (Link)

Mortarini,Roberta; Borri,Alessandra; Tragni,Gabrina; Bersani,Ilaria; Vegetti,Claudia; Bajetta,Emilio; Pilotti,Silvana; Cerundolo,Vincenzo; Anichini,Andrea. Peripheral Burst of Tumor-specific Cytotoxic T Lymphocytes and Infiltration of Metastatic Lesions by Memory CD8+ T Cells in Melanoma Patients Receiving Interleukin 12. Cancer Research 2000;60:3559-3568. (Link)

Defrere,Sylvie; Van Langendonckt,Anne; Moulin,Pierre; Befahy,Philippe; Gonzalez,Dolores; Martinez-Madrid,Belen; Dolmans,Marie Madeleine; Donnez,Jacques. Human Endometrial Epithelial Cells (EEC) Constitutively Express More Intercellular Adhesion Molecule (ICAM)-1 than Endometrial Stromal Cells (ESC) in Culture. American Journal of Reproductive Immunology 2005;54:5-12. (Link)

Mangioni,S.; Vigano,P.; Florio,P.; Borghi,O.; Vignali,M.; Petraglia,F.; Blasio,A.M.D.. Effect of activin A on tumor necrosis factor-. European Journal of Obstetrics and Gynecology 2005;123:218-223. (Link)

Suzuki,Takaaki; Ito,Tsutomu; Kashima,Ichiro; Teruya,Koji; Fukuda,Toyoki. Continuous perfusion of pulmonary arteries during total cardiopulmonary bypass favorably affects levels of circulating adhesion molecules and lung function. Journal of Thoracic and Cardiovascular Surgery 2001;122:242-248. (Link)

Fernandez-Real,Jose Manuel; Castro,Antoni; Vazquez,Gabriel; Casamitjana,Roser; Lopez-Bermejo,Abel; Penarroja,Georgina; Ricart,Wifredo. Adiponectin Is Associated With Vascular Function Independent of Insulin Sensitivity. Diabetes Care 2004;27:739-745. (Link)

Brun,Elisabetia; Zoppini,Giacomo; Zamboni,Cristina; Bonora,Enzo; Muggeo,Michele. Glucose Instability Is Associated With a High Level of Circulating P-Selectin. Diabetes Care 2001;24:1685(Link)

Arenillas,Juan F.; Alvarez-Sabin,Jose; Molina,Carlos A.; Chacon,Pilar; Fernandez-Cadenas,Israel; Ribo,Marc; Delgado,Pilar; Rubiera,Marta; Penalba,Anna; Rovira,Alex; Montaner,Joan. Progression of Symptomatic Intracranial Large Artery Atherosclerosis Is Associated With a Proinflammatory State and Impaired Fibrinolysis. Stroke 2008;39:1456-1463. (Link)

Vazquez-Agell,Monica; Sacanella,Emilio; Tobias,Ester; Monagas,Maria; Antunez,Emilia; Zamora-Ros,Raul; Andres-Lacueva,Cristina; Lamuela-Raventos,Rosa Ma; Fernandez-Sola,Joaquim; Nicolas,Jose Ma; Estruch,Ramon. Inflammatory Markers of Atherosclerosis Are Decreased after Moderate Consumption of Cava (Sparkling Wine) in Men with Low Cardiovascular Risk. The Journal of Nutrition 2007;137:2279-2284. (Link)

Fassbender,Klaus; Bertsch,Thomas; Mielke,Orell; Muhlhauser,Frank; Hennerici,Michael. Adhesion Molecules in Cerebrovascular Diseases : Evidence for an Inflammatory Endothelial Activation in Cerebral Large- and Small-Vessel Disease. Stroke 1999;30:1647-1650. (Link)

Kaufmann,P.; Smolle,K.H.; Brunner,G.A.; Demel,U.; Tilz,G.P.; Krejs,G.J.. Relation of serial measurements of plasma-soluble intercellular adhesion molecule-1 to severity of acute pancreatitis. The American Journal of Gastroenterology 1999;94:2412-2416. (Link)

VIGANO,P.A.O.L.; Di Blasio,Anna Maria; SOMIGLIANA,E.D.G.A.; COZZOLINO,S.A.B.R.; CANDIANI,M.A.S.S.; VIGNALI,M.A.R.I.. Suppression of Natural Killer Cell Function and Production of Soluble ICAM-1: Endometrial Stroma Versus Melanoma. American Journal of Reproductive Immunology 2001;46:342-348. (Link)

WHITCUP,SCOTT M.; VISTICA,BARBARA P.; MAGONE,M.TERESA; GEORGE,ROGER K.. Elevated serum levels of soluble ICAM-1 in uveitis patients predict underlying systemic disease. British Journal of Ophthalmology 1999;83:252a(Link)

Eschen,O.; Christensen,J.H.; De Caterina,R.; Schmidt,E.B.. Soluble adhesion molecules in healthy subjects: a dose-response study using n-3 fatty acids. Nutrition, Metabolism and Cardiovascular Diseases 2004;14:180-185. (Link)

Montaner,Joan; Rovira,Alex; Molina,Carlos; Arenillas,Juan; Ribo,Marc; Chacon,Pilar; Monasterio,Jasone; varez-Sabin,Jose. Plasmatic Level of Neuroinflammatory Markers Predict the Extent of Diffusion-Weighted Image Lesions in Hyperacute Stroke. J Cereb Blood Flow Metab 2003;23:1403-1407. (Link)

Dusitanond,P.; Eikelboom,J.W.; Hankey,G.J.; Thom,J.; Gilmore,G.; Loh,K.; Yi,Q.; Klijn,C.J.M.; Langton,P.; van Bockxmeer,F.M.; Baker,R.; Jamrozik,K.. Homocysteine-Lowering Treatment With Folic Acid, Cobalamin, and Pyridoxine Does Not Reduce Blood Markers of Inflammation, Endothelial Dysfunction, or Hypercoagulability in Patients With Previous Transient Ischemic Attack or Stroke: A Randomized Substudy of the VITATOPS Trial. Stroke 2005;36:144-146. (Link)

Valable,Samuel; Montaner,Joan; Bellail,Anita; Berezowski,Vincent; Brillault,Julien; Cecchelli,Romeo; Divoux,Didier; MacKenzie,Eric; Bernaudin,Myriam; Roussel,Simon; Petit,Edwige. VEGF-induced BBB permeability is associated with an MMP-9 activity increase in cerebral ischemia: both effects decreased by Ang-1. J Cereb Blood Flow Metab 2005;25:1491-1504. (Link)

References: Rothlein,R.; Mainolfi,E.A.; Czajkowski,M.; Marlin,S.D.. A form of circulating ICAM-1 in human serum. J.Immunol. 1991;147:3788-3793. (Link)

Rothlein,R.; Mainolfi,E.A.; Czajkowski,M.; Marlin,S.D.. A form of circulating ICAM-1 in human serum. J.Immunol. 1991;147:3788-3793. (Link)

Altomonte,M.; Colizzi,F.; Esposito,G.; Maio,M.. Circulating intercellular adhesion molecule 1 as a marker of disease progression in cutaneous melanoma. N Engl.J.Med. 1992;327:959(Link)

Altomonte,M.; Colizzi,F.; Esposito,G.; Maio,M.. Circulating intercellular adhesion molecule 1 as a marker of disease progression in cutaneous melanoma. N Engl.J.Med. 1992;327:959(Link)

Salafia,C.M.; DeVore,G.R.; Mainolfi,E.; Kelly,J.; Pezzullo,J.C.; Rothlein,R.. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am.J.Obstet.Gynecol. 1993;830-834. (Link)

Salafia,C.M.; DeVore,G.R.; Mainolfi,E.; Kelly,J.; Pezzullo,J.C.; Rothlein,R.. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am.J.Obstet.Gynecol. 1993;830-834. (Link)

Pober,J.S.; Gimbrone,M.A.,Jr.; Lapierre,L.A.; Mendrick,D.L.; Fiers,W.; Rothlein,R.; Springer,T.A.. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. J.Immunol. 1986;137:1893-1896. (Link)

Pober,J.S.; Gimbrone,M.A.,Jr.; Lapierre,L.A.; Mendrick,D.L.; Fiers,W.; Rothlein,R.; Springer,T.A.. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. J.Immunol. 1986;137:1893-1896. (Link)

Dustin,M.L.; Rothlein,R.; Bhan,A.K.; Dinarello,C.A.; Springer,T.A.. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J.Immunol. 1986;137:245-254. (Link)

Dustin,M.L.; Rothlein,R.; Bhan,A.K.; Dinarello,C.A.; Springer,T.A.. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J.Immunol. 1986;137:245-254. (Link)

Lampeter,E.R.; Kishimoto,T.K.; Rothlein,R.; Mainolfi,E.A.; Bertrams,J.; Kolb,H.; Martin,S.. Elevated levels of circulating adhesion molecules in IDDM patients and in subjects at risk for IDDM. Diabetes 1992;41:1668-1671. (Link)

Lampeter,E.R.; Kishimoto,T.K.; Rothlein,R.; Mainolfi,E.A.; Bertrams,J.; Kolb,H.; Martin,S.. Elevated levels of circulating adhesion molecules in IDDM patients and in subjects at risk for IDDM. Diabetes 1992;41:1668-1671. (Link)

Vejlsgaard,G.L.; Ralfkiaer,E.; Avnstorp,C.; Czajkowski,M.; Marlin,S.D.; Rothlein,R.. Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin lesions and malignant cutaneous lymphomas. J.Am.Acad.Dermatol. 1989;20:782-790. (Link)

Vejlsgaard,G.L.; Ralfkiaer,E.; Avnstorp,C.; Czajkowski,M.; Marlin,S.D.; Rothlein,R.. Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin lesions and malignant cutaneous lymphomas. J.Am.Acad.Dermatol. 1989;20:782-790. (Link)

Lal,R.B.; Mainolfi,E.; Rothlein,R.. Elevated levels of cICAM-1 in patients with human T-cell leukemia virus type I associated myelopathy and adult T-cell leukemia. Blood 1992;80:2434-2435. (Link)

Lal,R.B.; Mainolfi,E.; Rothlein,R.. Elevated levels of cICAM-1 in patients with human T-cell leukemia virus type I associated myelopathy and adult T-cell leukemia. Blood 1992;80:2434-2435. (Link)

Salafia,C.M.; DeVore,G.R.; Mainolfi,E.; Kelly,J.; Pezzullo,J.C.; Rothlein,R.. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am.J.Obstet.Gynecol. 1993;169:830-834. (Link)

Salafia,C.M.; DeVore,G.R.; Mainolfi,E.; Kelly,J.; Pezzullo,J.C.; Rothlein,R.. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am.J.Obstet.Gynecol. 1993;169:830-834. (Link)

Most,J.; Zangerle,R.; Herold,M.; Fuchs,D.; Wachter,H.; Fritsch,P.; Dierich,M.P.. Elevated concentrations of circulating intercellular adhesion molecule 1 (ICAM-1) in HIV-1 infection. J.Acquir.Immune Defic.Syndr. 1993;6:221-226. (Link)

Most,J.; Zangerle,R.; Herold,M.; Fuchs,D.; Wachter,H.; Fritsch,P.; Dierich,M.P.. Elevated concentrations of circulating intercellular adhesion molecule 1 (ICAM-1) in HIV-1 infection. J.Acquir.Immune Defic.Syndr. 1993;6:221-226. (Link)

Lisby,S.; Ralfkiaer,E.; Rothlein,R.; Vejlsgaard,G.L.. Intercellular adhesion molecule-I (ICAM-I) expression correlated to inflammation. Br.J.Dermatol. 1989;120:479-484. (Link)

Lisby,S.; Ralfkiaer,E.; Rothlein,R.; Vejlsgaard,G.L.. Intercellular adhesion molecule-I (ICAM-I) expression correlated to inflammation. Br.J.Dermatol. 1989;120:479-484. (Link)

Rothlein,R.; Dustin,M.L.; Marlin,S.D.; Springer,T.A.. A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J.Immunol. 1986;137:1270-1274. (Link)

Harning,R.; Mainolfi,E.; Bystryn,J.C.; Henn,M.; Merluzzi,V.J.; Rothlein,R.. Serum levels of circulating intercellular adhesion molecule 1 in human malignant melanoma. Cancer Res. 1991;51:5003-5005. (Link)

Harning,R.; Mainolfi,E.; Bystryn,J.C.; Henn,M.; Merluzzi,V.J.; Rothlein,R.. Serum levels of circulating intercellular adhesion molecule 1 in human malignant melanoma. Cancer Res. 1991;51:5003-5005. (Link)

View More Products

AntigensArea of BiologyCell TypeFormats
Recently Viewed Products

  1. Human sHER-2 Instant ELISA

  2. Human sHER-2 Instant ELISA

  3. Human sE-selectin Instant ELISA

  4. Human sE-selectin Instant ELISA

  5. Human sAPO-1/Fas FlowCytomix Simplex

Related Products

Home | Sitemap

Copyright © 2000-2011 eBioscience, Inc.

Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.