Human sICAM-1 Instant ELISA

Description: The human sICAM-1 INSTANT CE marked ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.

The addition of sample is all that is required to start the assay. The Instant ELISA plates contain coating antibody, lyophilized detection antibody, streptavidin-HRP and sample diluent. Wells containing the ready-to-use standard curve are provided separately.

ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center Bcells and dendritic cells in tonsils, lymph nodes and Peyer's patches.

ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFNg within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.

sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation.

Citations: Beeh,Kai M.; Beier,Jutta; Kornmann,Oliver; Mander,Annette; Buhl,Roland. Long-term Repeatability of Induced Sputum Cells and Inflammatory Markers in Stable, Moderately Severe COPD. Chest 2003;123:778-783. (Link)

Triolo; Triolo; Accardo,Palumbo; Carbone; Ferrante; Casiglia; Giardina. IgG anti-endothelial cell antibodies (AECA) in type I diabetes mellitus; induction of adhesion molecule expression in cultured endothelial cells. Clinical and Experimental Immunology 1998;111:491-496. (Link)

Piconi,L.; Quagliaro,L.; Da Ros,R.; Assaloni,R.; Giugliano,D.; Esposito,K.; Szabo,C.; Ceriello,A.. Intermittent high glucose enhances ICAM-1, VCAM-1, E-selectin and interleukin-6 expression in human umbilical endothelial cells in culture: the role of poly(ADP-ribose) polymerase. Journal of Thrombosis and Haemostasis 2004;2:1453-1459. (Link)

Chan,Loretta; Leung,Joseph; Tsang,Anita; Tang,Sydney; Lai,Kar. Activation of tubular epithelial cells by mesangial-derived TNF-[alpha]: Glomerulotubular communication in IgA nephropathy. Kidney Int 2005;67:602-612. (Link)

Chan J.C.N.,J C K Cheung,C D A Stehouwer,J J Emeis,P C Y Tong,G T C Ko and J S Yudkin. The central roles of obesity-associated dyslipidaemia, endothelial activation and cytokines in the Metabolic Syndromean analysis by structural equation modelling. International Journal of Obesity 2002;26:994-1008. (Link)

Chin,K.; Nakamura,T.; Shimizu,K.; Mishima,M.; Nakamura,T.; Miyasaka,M.; Ohi,M.. Effects of nasal continuous positive airway pressure on soluble cell adhesion molecules in patients with obstructive... The American Journal of Medicine 2000;109:562-567. (Link)

Caproni,M.; Volpi,W.; Giomi,B.; Torchia,D.; Del Bianco,E.; Fabbri,P.. Cellular adhesion molecules in chronic urticaria: modulation of serum levels occurs during levocetirizine treatment. British Journal of Dermatology 2006;155:1270-1274. (Link)

Zabad,R.K.; Metz,L.M.; Todoruk,T.R.; Zhang,Y.; Mitchell,J.R.; Yeung,M.; Patry,D.G.; Bell,R.B.; Yong,V.W.. The clinical response to minocycline in multiple sclerosis is accompanied by beneficial immune changes: a pilot study. Multiple Sclerosis 2007;13:517-526. (Link)

Reindl,M.; Lutterotti,A.; Ingram,J.; Schanda,K.; Gassner,C.; Deisenhammer,F.; Berger,T.; Lorenz,E.. Mutations in the gene for toll-like receptor 4 and multiple sclerosis. Tissue Antigens 2003;61:85-88. (Link)

Hammett,Christopher J.K.; Prapavessis,Harry; Baldi,J.Chris; Varo,Nerea; Schoenbeck,Uwe; Ameratunga,Rohan; French,John K.; White,Harvey D.; Stewart,Ralph A.H.. Effects of exercise training on 5 inflammatory markers associated with cardiovascular risk. American Heart Journal 2006;151:(Link)

Leonardi,A.; Abelson,M.B.. Double-masked, randomized, placebo-controlled clinical study of the mast cell-stabilizing effects of treatment with... Clinical Therapeutics 2003;25:2539-2552. (Link)

Stefanska,Anna; Sypniewskay,Grazyna; Senterkiewicz,Lilla. Inflammatory Markers and Cardiovascular Risk in Healthy Polish Women across the Menopausal Transition. Clinical Chemistry 2005;51:1893-1895. (Link)

Mortarini,Roberta; Borri,Alessandra; Tragni,Gabrina; Bersani,Ilaria; Vegetti,Claudia; Bajetta,Emilio; Pilotti,Silvana; Cerundolo,Vincenzo; Anichini,Andrea. Peripheral Burst of Tumor-specific Cytotoxic T Lymphocytes and Infiltration of Metastatic Lesions by Memory CD8+ T Cells in Melanoma Patients Receiving Interleukin 12. Cancer Research 2000;60:3559-3568. (Link)

Defrere,Sylvie; Van Langendonckt,Anne; Moulin,Pierre; Befahy,Philippe; Gonzalez,Dolores; Martinez-Madrid,Belen; Dolmans,Marie Madeleine; Donnez,Jacques. Human Endometrial Epithelial Cells (EEC) Constitutively Express More Intercellular Adhesion Molecule (ICAM)-1 than Endometrial Stromal Cells (ESC) in Culture. American Journal of Reproductive Immunology 2005;54:5-12. (Link)

Buttmann,Mathias; Lorenz,Alexander; Weishaupt,Andreas; Rieckmann,Peter. Atorvastatin partially prevents an inflammatory barrier breakdown of cultured human brain endothelial cells at a pharmacologically relevant concentration. Journal of Neurochemistry 2007;102:1001-1008. (Link)

Mangioni,S.; Vigano,P.; Florio,P.; Borghi,O.; Vignali,M.; Petraglia,F.; Blasio,A.M.D.. Effect of activin A on tumor necrosis factor-. European Journal of Obstetrics and Gynecology 2005;123:218-223. (Link)

Suzuki,Takaaki; Ito,Tsutomu; Kashima,Ichiro; Teruya,Koji; Fukuda,Toyoki. Continuous perfusion of pulmonary arteries during total cardiopulmonary bypass favorably affects levels of circulating adhesion molecules and lung function. Journal of Thoracic and Cardiovascular Surgery 2001;122:242-248. (Link)

Klimiuk,P.A.; Sierakowski,S.; Latosiewicz,R.; Cylwik,J.P.; Cylwik,B.; Skowronski,J.; Chwiecko,J.. Soluble adhesion molecules (ICAM-1, VCAM-1, and E-selectin) and vascular endothelial growth factor (VEGF) in patients with distinct variants of rheumatoid synovitis. Annals of the Rheumatic Diseases 2002;61:804-809. (Link)

Fernandez-Real,Jose Manuel; Castro,Antoni; Vazquez,Gabriel; Casamitjana,Roser; Lopez-Bermejo,Abel; Penarroja,Georgina; Ricart,Wifredo. Adiponectin Is Associated With Vascular Function Independent of Insulin Sensitivity. Diabetes Care 2004;27:739-745. (Link)

Brun,Elisabetia; Zoppini,Giacomo; Zamboni,Cristina; Bonora,Enzo; Muggeo,Michele. Glucose Instability Is Associated With a High Level of Circulating P-Selectin. Diabetes Care 2001;24:1685(Link)

Gomez,Jose Manuel; Sahun,Manel; Vila,Ramon; Domenech,Pere; Catalina,Pablo; Soler,Juan; Badimon,Lina. Peripheral fibrinolytic markers, soluble adhesion molecules, inflammatory cytokines and endothelial function in hypopituitary adults with growth hormone deficiency. Clinical Endocrinology 2006;64:632-639. (Link)

Arenillas,Juan F.; Alvarez-Sabin,Jose; Molina,Carlos A.; Chacon,Pilar; Fernandez-Cadenas,Israel; Ribo,Marc; Delgado,Pilar; Rubiera,Marta; Penalba,Anna; Rovira,Alex; Montaner,Joan. Progression of Symptomatic Intracranial Large Artery Atherosclerosis Is Associated With a Proinflammatory State and Impaired Fibrinolysis. Stroke 2008;39:1456-1463. (Link)

Vazquez-Agell,Monica; Sacanella,Emilio; Tobias,Ester; Monagas,Maria; Antunez,Emilia; Zamora-Ros,Raul; Andres-Lacueva,Cristina; Lamuela-Raventos,Rosa Ma; Fernandez-Sola,Joaquim; Nicolas,Jose Ma; Estruch,Ramon. Inflammatory Markers of Atherosclerosis Are Decreased after Moderate Consumption of Cava (Sparkling Wine) in Men with Low Cardiovascular Risk. The Journal of Nutrition 2007;137:2279-2284. (Link)

Fassbender,Klaus; Bertsch,Thomas; Mielke,Orell; Muhlhauser,Frank; Hennerici,Michael. Adhesion Molecules in Cerebrovascular Diseases : Evidence for an Inflammatory Endothelial Activation in Cerebral Large- and Small-Vessel Disease. Stroke 1999;30:1647-1650. (Link)

Kaufmann,P.; Smolle,K.H.; Brunner,G.A.; Demel,U.; Tilz,G.P.; Krejs,G.J.. Relation of serial measurements of plasma-soluble intercellular adhesion molecule-1 to severity of acute pancreatitis. The American Journal of Gastroenterology 1999;94:2412-2416. (Link)

References: Rothlein,R.; Mainolfi,E.A.; Czajkowski,M.; Marlin,S.D.. A form of circulating ICAM-1 in human serum. J.Immunol. 1991;147:3788-3793. (Link)

Altomonte,M.; Colizzi,F.; Esposito,G.; Maio,M.. Circulating intercellular adhesion molecule 1 as a marker of disease progression in cutaneous melanoma. N Engl.J.Med. 1992;327:959(Link)

Salafia,C.M.; DeVore,G.R.; Mainolfi,E.; Kelly,J.; Pezzullo,J.C.; Rothlein,R.. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am.J.Obstet.Gynecol. 1993;830-834. (Link)

Pober,J.S.; Gimbrone,M.A.,Jr.; Lapierre,L.A.; Mendrick,D.L.; Fiers,W.; Rothlein,R.; Springer,T.A.. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. J.Immunol. 1986;137:1893-1896. (Link)

Dustin,M.L.; Rothlein,R.; Bhan,A.K.; Dinarello,C.A.; Springer,T.A.. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J.Immunol. 1986;137:245-254. (Link)

Lampeter,E.R.; Kishimoto,T.K.; Rothlein,R.; Mainolfi,E.A.; Bertrams,J.; Kolb,H.; Martin,S.. Elevated levels of circulating adhesion molecules in IDDM patients and in subjects at risk for IDDM. Diabetes 1992;41:1668-1671. (Link)

Vejlsgaard,G.L.; Ralfkiaer,E.; Avnstorp,C.; Czajkowski,M.; Marlin,S.D.; Rothlein,R.. Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin lesions and malignant cutaneous lymphomas. J.Am.Acad.Dermatol. 1989;20:782-790. (Link)

Lal,R.B.; Mainolfi,E.; Rothlein,R.. Elevated levels of cICAM-1 in patients with human T-cell leukemia virus type I associated myelopathy and adult T-cell leukemia. Blood 1992;80:2434-2435. (Link)

Salafia,C.M.; DeVore,G.R.; Mainolfi,E.; Kelly,J.; Pezzullo,J.C.; Rothlein,R.. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am.J.Obstet.Gynecol. 1993;169:830-834. (Link)

Most,J.; Zangerle,R.; Herold,M.; Fuchs,D.; Wachter,H.; Fritsch,P.; Dierich,M.P.. Elevated concentrations of circulating intercellular adhesion molecule 1 (ICAM-1) in HIV-1 infection. J.Acquir.Immune Defic.Syndr. 1993;6:221-226. (Link)

Lisby,S.; Ralfkiaer,E.; Rothlein,R.; Vejlsgaard,G.L.. Intercellular adhesion molecule-I (ICAM-I) expression correlated to inflammation. Br.J.Dermatol. 1989;120:479-484. (Link)

Rothlein,R.; Dustin,M.L.; Marlin,S.D.; Springer,T.A.. A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J.Immunol. 1986;137:1270-1274. (Link)

Harning,R.; Mainolfi,E.; Bystryn,J.C.; Henn,M.; Merluzzi,V.J.; Rothlein,R.. Serum levels of circulating intercellular adhesion molecule 1 in human malignant melanoma. Cancer Res. 1991;51:5003-5005. (Link)

Adams,D.H.; Hubscher,S.G.; Shaw,J.; Rothlein,R.; Neuberger,J.M.. Intercellular adhesion molecule 1 on liver allografts during rejection. Lancet 1989;2:1122-1125. (Link)

Potocnik,A.J.; Kinne,R.; Menninger,H.; Zacher,J.; Emmrich,F.; Kroczek,R.A.. Expression of activation antigens on T cells in rheumatoid arthritis patients. Scand.J.Immunol. 1990;31:213-224. (Link)

Becker,J.C.; Dummer,R.; Schwinn,A.; Hartmann,A.A.; Burg,G.. Circulating intercellular adhesion molecule-1 in melanoma patients: induction by interleukin-2 therapy. J.Immunother.(1991.) 1992;12:147-150. (Link)

Marlin,S.D.; Springer,T.A.. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell 1987;51:813-819. (Link)

Adams,D.H.; Mainolfi,E.; Elias,E.; Neuberger,J.M.; Rothlein,R.. Detection of circulating intercellular adhesion molecule-1 after liver transplantation--evidence of local release within the liver during graft rejection. Transplantation 1993;55:83-87. (Link)

Rothlein,R.; Czajkowski,M.; O'Neill,M.M.; Marlin,S.D.; Mainolfi,E.; Merluzzi,V.J.. Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies. J.Immunol. 1988;141:1665-1669. (Link)

Wegner,C.D.; Gundel,R.H.; Reilly,P.; Haynes,N.; Letts,L.G.; Rothlein,R.. Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science 1990;247:456-459. (Link)