Human sCD44var (v6) Instant ELISA

Also known as: VFF-7 Phagocytic glycoprotein I, Hermes antigen

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS210INST*

Cat. No. Size
BMS210INST 128 tests
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Data for Human sCD44var (v6) Instant ELISA.

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  • Data for Human sCD44var (v6) Instant ELISA.
Description

Description: The human sCD44var (v6) Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD44var (v6). The human sCD44var (v6) Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Alternative splicing of the mRNA gives rise to the different isoforms, containing inserts of varying sizes in the extracellular domain of the molecule (exons v2-v10). All CD44 isoforms are variably glycosylated. In contrast to standard CD44 (CD44std) which is almost ubiquitously expressed, the variety of CD44 isoforms (CD44var) have a much more restricted distribution, e.g., on activated lymphocytes and macrophages (exon v6).

The sCD44var(v6) ELISA detects all circulating CD44 isoforms comprising the sCD44var(v6) sequences.

Determination of sCD44var(v6) will provide more detailed insight into different pathological modifications during cancer and other diseases.

Details
Reactivity Human
Sample Volume 20 uL (1:20 prediluted)
Suitable Sample Types cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine
Sensitivity 0.06 ng/mL
Standard Curve Range 0.16 - 10.0 ng/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD44var (v6), HRP-Conjugate (human sCD44var (v6) monoclonal antibody) and Sample Diluent, lyophilized
Aluminium pouch(es) with a human sCD44var (v6) Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Sample Diluent
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Reported Applications ELISA
Documentation
TDS Link Download TDS
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References

Citations: Fuchs,S.; Rolauffs,B.; Arndt,S.; Tibesku,C.O.; Prehm,P.. CD44H and the isoforms CD44v5 and CD44v6 in the synovial fluid of the osteoarthritic human knee joint. Osteoarthritis and Cartilage 2003;11:839-844. (Link)

Loeffler-Ragg,J.; Steurer,M.; Ulmer,H.; Skvortsov,S.; Kircher,B.; Herold,M.; Zwierzina,H.; Stauder,R.. Elevated levels of serum CD44 and E-cadherin predict an unfavourable outcome in myelodysplastic syndromes. Leukemia 2006;20:2064-2067. (Link)

Van Hal,Nicole L.W.; van Dongen,Guus A.M.S.; Ten Brink,Corlinda B.M.; Heider,Karl Heinz; Rech-Weichselbraun,Irene; Snow,Gordon B.; Brakenhoff,Ruud H.. Evaluation of Soluble CD44v6 as a Potential Serum Marker for Head and Neck Squamous Cell Carcinoma. Clinical Cancer Research 1999;5:3534-3541. (Link)

Braumller,H.; Gansauge,S.; Ramadani,M.; Gansauge,F.. CD44v6 cell surface expression is a common feature of macrophages and macrophage-like cells - implication for a natural... FEBS Letters 2000;476:240-247. (Link)

Kanke,Minoru; Fujii,Masato; Kameyama,Kaori; Kanzaki,Jin; Tokumaru,Yutaka; Imanishi,Yorihisa; Tomita,Toshiki; Matsumura,Yasuhiro. Role of CD44 Variant Exon 6 in Invasion of Head and Neck Squamous Cell Carcinoma. Archives of Otolaryngology - Head and Neck Surgery 2000;126:1217-1223. (Link)

Ekici,Sinan; Ayhan,Ay; Kendi,Sezer; Ozen,Haluk. Determination of prognosis in patients with prostate cancer treated with radical prostatectomy: prognostic value of CD44v6 score. The Journal of urology 2002;167:2037-2041. (Link)

Scott,David A.; Stapleton,John A.; Palmer,Richard M.; Wilson,Ron F.; Sutherland,Gay; Coward,Paula Y.; Gustavsson,Gunnar; Odell,Edward W.; Poston,Robin N.. Plasma Concentrations of Reputed Tumor-associated Soluble CD44 Isoforms (v5 and v6) in Smokers Are Dose Related and Decline on Smoking Cessation. Cancer Epidemiology Biomarkers & Prevention 2000;9:1211-1214. (Link)

Afify,Alaa; Lynne,Lin Chang; Howell,Lydia. Correlation of cytologic examination with ELISA assays for hyaluronan and soluble CD44v6 levels in evaluation of effusions. Diagnostic cytopathology 2007;35:105-110. (Link)

Stroomer,Joep W.G.; Roos,Jan C.; Sproll,Marlies; Quak,Jasper J.; Heider,Karl Heinz; Wilhelm,Bram J.; Castelijns,Jonas A.; Meyer,Reiner; Kwakkelstein,Marthin O.; Snow,Gordon B.; Adolf,Gunther R.; van Dongen,Guus A.M.S.. Safety and Biodistribution of 99mTechnetium-labeled Anti-CD44v6 Monoclonal Antibody BIWA 1 in Head and Neck Cancer Patients. Clinical Cancer Research 2000;6:3046-3055. (Link)

Palmer,R.M.; Stapleton,J.A.; Sutherland,G.; Coward,P.Y.; Wilson,R.F.; Scott,D.A.. Effect of nicotine replacement and quitting smoking on circulating adhesion molecule profiles (sICAM-1, sCD44v5, sCD44v6). European Journal of Clinical Investigation 2002;32:852-857. (Link)

Franzmann,Elizabeth J.; Reategui,Erika P.; Pedroso,Felipe; Pernas,Francisco G.; Karakullukcu,Baris M.; Carraway,Kermit L.; Hamilton,Kara; Singal,Rakesh; Goodwin,W.Jarrard. Soluble CD44 Is a Potential Marker for the Early Detection of Head and Neck Cancer. Cancer Epidemiology Biomarkers & Prevention 2007;16:1348-1355. (Link)

Afify,A.M.; Craig,S.; Paulino,A.F.G.; Stern,R.. Expression of hyaluronic acid and its receptors, CD44s and CD44v6, in normal, hyperplastic, and neoplastic endometrium. Annals of Diagnostic Pathology 2005;9:312-318. (Link)

References: Humbria,A.; az-Gonzalez,F.; Campanero,M.R.; Arroyo,A.G.; Laffon,A.; Gonzalez-Amaro,R.; Sanchez-Madrid,F.. Expression of L-selectin, CD43, and CD44 in synovial fluid neutrophils from patients with inflammatory joint diseases. Evidence for a soluble form of L-selectin in synovial fluid. Arthritis Rheum. 1994;37:342-348. (Link)

Arch,R.; Wirth,K.; Hofmann,M.; Ponta,H.; Matzku,S.; Herrlich,P.; Zoller,M.. Participation in normal immune responses of a metastasis-inducing splice variant of CD44. Science 1992;257:682-685. (Link)

Hofmann,M.; Rudy,W.; Zoller,M.; Tolg,C.; Ponta,H.; Herrlich,P.; Gunthert,U.. CD44 splice variants confer metastatic behavior in rats: homologous sequences are expressed in human tumor cell lines. Cancer Res. 1991;51:5292-5297. (Link)

Heider,K.H.; Dammrich,J.; Skroch-Angel,P.; Muller-Hermelink,H.K.; Vollmers,H.P.; Herrlich,P.; Ponta,H.. Differential expression of CD44 splice variants in intestinal- and diffuse-type human gastric carcinomas and normal gastric mucosa. Cancer Res. 1993;53:4197-4203. (Link)

Stamenkovic,I.; Aruffo,A.; Amiot,M.; Seed,B.. The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells. EMBO J. 1991;10:343-348. (Link)

Fujiwara,K.; Yoshino,T.; Miyake,K.; Ohara,N.; Akagi,T.. Expression of a lymphocyte adhesion molecule (CD44) in malignant lymphomas: relevance to primary site, histological subtype and clinical stage. Acta Med.Okayama 1993;47:215-222. (Link)

Moller,P.; Eichelmann,A.; Mechtersheimer,G.; Koretz,K.. Expression of beta 1-integrins, H-CAM (CD44) and LECAM-1 in primary gastro-intestinal B-cell lymphomas as compared to the adhesion receptor profile of the gut-associated lymphoid system, tonsil and peripheral lymph node. Int J.Cancer 1991;49:846-855. (Link)

Salmi,M.; Gron-Virta,K.; Sointu,P.; Grenman,R.; Kalimo,H.; Jalkanen,S.. Regulated expression of exon v6 containing isoforms of CD44 in man: downregulation during malignant transformation of tumors of squamocellular origin. J.Cell Biol. 1993;122:431-442. (Link)

Heider,K.H.; Hofmann,M.; Hors,E.; van den,Berg F.; Ponta,H.; Herrlich,P.; Pals,S.T.. A human homologue of the rat metastasis-associated variant of CD44 is expressed in colorectal carcinomas and adenomatous polyps. J.Cell Biol. 1993;120:227-233. (Link)

Gunthert,U.; Hofmann,M.; Rudy,W.; Reber,S.; Zoller,M.; Haussmann,I.; Matzku,S.; Wenzel,A.; Ponta,H.; Herrlich,P.. A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell 1991;65:13-24. (Link)

Kuppner,M.C.; Van,Meir E.; Gauthier,T.; Hamou,M.F.; de,Tribolet N.. Differential expression of the CD44 molecule in human brain tumours. Int.J.Cancer 1992;50:572-577. (Link)

Joensuu,H.; Ristamaki,R.; Klemi,P.J.; Jalkanen,S.. Lymphocyte homing receptor (CD44) expression is associated with poor prognosis in gastrointestinal lymphoma. Br.J.Cancer 1993;68:428-432. (Link)

Koopman,G.; Heider,K.H.; Horst,E.; Adolf,G.R.; van den,Berg F.; Ponta,H.; Herrlich,P.; Pals,S.T.. Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44. J.Exp.Med. 1993;177:897-904. (Link)

Matsumura,Y.; Tarin,D.. Significance of CD44 gene products for cancer diagnosis and disease evaluation. Lancet 1992;340:1053-1058. (Link)

Guo,M.M.; Hildreth,J.E.. HIV-induced loss of CD44 expression in monocytic cell lines. J.Immunol. 1993;151:2225-2236. (Link)

Omary,M.B.; Trowbridge,I.S.; Letarte,M.; Kagnoff,M.F.; Isacke,C.M.. Structural heterogeneity of human Pgp-1 and its relationship with p85. Immunogenetics 1988;27:460-464. (Link)


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