Human sCD40L Platinum ELISA (extra sensitive)

Description: The human sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD40L. The human sCD40L ELISA is for research use only. Not for diagnostic or therapeutic procedures.

CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.)

A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40.

CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40 L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases.

Citations: Garlichs,C.D.; Kozina,S.; Fateh-Moghadam,S.; Tomandl,B.; Stumpf,C.; Eskafi,S.; Raaz,D.; Schmeisser,A.; Yilmaz,A.; Ludwig,J.; Neundorfer,B.; Daniel,W.G.. Upregulation of CD40-CD40 Ligand (CD154) in Patients With Acute Cerebral Ischemia. Stroke 2003;34:1412-1418. (Link)

Viallard,Jean Francois; Solanilla,Anne; Gauthier,Bruno; Contin,Cecile; Dechanet,Julie; Grosset,Christophe; Moreau,Jean Francois; Praloran,Vincent; Nurden,Paquita; Pellegrin,Jean Luc; Nurden,Alan T.; Ripoche,Jean. Increased soluble and platelet-associated CD40 ligand in essential thrombocythemia and reactive thrombocytosis. Blood 2002;99:2612-2614. (Link)

Aukrust,Pal; Muller,Fredrik; Ueland,Thor; Berget,Trude; Aaser,Elinor; Brunsvig,Anne; Solum,Nils Olav; Forfang,Kolbjorn; Froland,Stig S.; Gullestad,Lars. Enhanced Levels of Soluble and Membrane-Bound CD40 Ligand in Patients With Unstable Angina : Possible Reflection of T Lymphocyte and Platelet Involvement in the Pathogenesis of Acute Coronary Syndromes. Circulation 1999;100:614-620. (Link)

Damas,Jan K.; Jensenius,Mogens; Ueland,Thor; Otterdal,Kari; Yndestad,Arne; Froland,Stig S.; Rolain,Jean Marc; Myrvang,Bjorn; Raoult,Didier; Aukrust,Pal. Increased Levels of Soluble CD40L in African Tick Bite Fever: Possible Involvement of TLRs in the Pathogenic Interaction between Rickettsia africae, Endothelial Cells, and Platelets. The Journal of Immunology 2006;177:2699-2706. (Link)

Hetzel,Jurgen; Balletshofer,Bernd; Rittig,Kilian; Walcher,Daniel; Kratzer,Wolfgang; Hombach,Vinzenz; Haring,Hans Ulrich; Koenig,Wolfgang; Marx,Nikolaus. Rapid Effects of Rosiglitazone Treatment on Endothelial Function and Inflammatory Biomarkers. Arteriosclerosis, Thrombosis, and Vascular Biology 2005;25:1804-1809. (Link)

de Lemos,James A.; Zirlik,Andreas; Schonbeck,Uwe; Varo,Nerea; Murphy,Sabina A.; Khera,Amit; McGuire,Darren K.; Stanek,Greg; Lo,Hao S.; Nuzzo,Rebecca; Morrow,David A.; Peshock,Ronald; Libby,Peter. Associations Between Soluble CD40 Ligand, Atherosclerosis Risk Factors, and Subclinical Atherosclerosis: Results from the Dallas Heart Study. Arteriosclerosis, Thrombosis, and Vascular Biology 2005;25:2192-2196. (Link)

Vazquez-Agell,Monica; Sacanella,Emilio; Tobias,Ester; Monagas,Maria; Antunez,Emilia; Zamora-Ros,Raul; Andres-Lacueva,Cristina; Lamuela-Raventos,Rosa Ma; Fernandez-Sola,Joaquim; Nicolas,Jose Ma; Estruch,Ramon. Inflammatory Markers of Atherosclerosis Are Decreased after Moderate Consumption of Cava (Sparkling Wine) in Men with Low Cardiovascular Risk. The Journal of Nutrition 2007;137:2279-2284. (Link)

Nagasawa,Masayuki; Zhu,Yi; Isoda,Takeshi; Tomizawa,Daisuke; Itoh,Sukeyuki; Kajiwara,Michiko; Morio,Tomohiro; Nonoyama,Shigeaki; Shimizu,Norio; Mizutani,Shuki. Analysis of serum soluble CD40 ligand (sCD40L) in the patients undergoing allogeneic stem cell transplantation: platelet is a major source of serum sCD40L. European Journal of Haematology 2005;74:54-60. (Link)

Schernthaner,G.H.; Kopp,H.P.; Krzyzanowska,K.; Kriwanek,S.; Koppensteiner,R.; Schernthaner,G.. Soluble CD40L in patients with morbid obesity: significant reduction after bariatric surgery. European Journal of Clinical Investigation 2006;36:395-401. (Link)

Li,Jiang; Zhao,Shui Ping; Peng,Dao quan; Xu,Zhu mei; Zhou,Hong nian. Early Effect of Pravastatin on Serum Soluble CD40L, Matrix Metalloproteinase-9, and C-Reactive Protein in Patients with Acute Myocardial Infarction. Clinical Chemistry 2004;50:1696-1699. (Link)

Dusitanond,P.; Eikelboom,J.W.; Hankey,G.J.; Thom,J.; Gilmore,G.; Loh,K.; Yi,Q.; Klijn,C.J.M.; Langton,P.; van Bockxmeer,F.M.; Baker,R.; Jamrozik,K.. Homocysteine-Lowering Treatment With Folic Acid, Cobalamin, and Pyridoxine Does Not Reduce Blood Markers of Inflammation, Endothelial Dysfunction, or Hypercoagulability in Patients With Previous Transient Ischemic Attack or Stroke: A Randomized Substudy of the VITATOPS Trial. Stroke 2005;36:144-146. (Link)

Szegedi,A.; Irinyi,B.; Gal,M.; Hunyadi,J.; Danko,K.; Kiss,E.; Sipka,S.; Szegedi,G.; Gyimesi,E.. Significant correlation between the CD63 assay and the histamine release assay in chronic urticaria. British Journal of Dermatology 2006;155:67-75. (Link)

Mayo,Marlyn J.; Mosby,James M.; Jeyarajah,Rohan; Combes,Burton; Khilnani,Smina; Al-halimi,Maha; Handem,Iorna; Grammer,Amrie C.; Lipsky,Peter E.. The relationship between hepatic immunoglobulin production and CD154 expression in chronic liver diseases. Liver International 2006;26:187-196. (Link)

Schonbeck,Uwe; Gerdes,Norbert; Varo,Nerea; Reynolds,Rebecca S.; Horton,Daniel B.; Bavendiek,Udo; Robbie,Linda; Ganz,Peter; Kinlay,Scott; Libby,Peter. Oxidized Low-Density Lipoprotein Augments and 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Inhibitors Limit CD40 and CD40L Expression in Human Vascular Cells. Circulation 2002;106:2888-2893. (Link)

Stellbaum,C.; Willich,T.; Boltzen,U.; Ayral,Y.; Szotowski,B.; Piorkowski,M.; Schultheiss,H.P.; Rauch,U.. Clopidogrel-mediated reduction of circulating tissue factor in patients with stable coronary artery disease. European Journal of Haematology 2007;78:347-352. (Link)

Cruden,N.L.M.; Graham,C.; Harding,S.A.; Ludlam,C.A.; Fox,K.A.A.; Newby,D.E.. Plasma TAFI and soluble CD40 ligand do not predict reperfusion following thrombolysis for acute myocardial infarction. Thrombosis Research 2006;118:189-197. (Link)

Sacanella,Emilio; Vazquez-Agell,Monica; Mena,Mari Pau; Antunez,Emilia; Fernandez-Sola,Joaquim; Nicolas,Jose Maria; Lamuela-Raventos,Rosa M.; Ros,Emilio; Estruch,Ramon. Down-regulation of adhesion molecules and other inflammatory biomarkers after moderate wine consumption in healthy women: a randomized trial. American Journal of Clinical Nutrition 2007;86:1463-1469. (Link)

Tsioufis,C.; Dimitriadis,K.; Taxiarchou,E.; Vasiliadou,C.; Chartzoulakis,G.; Tousoulis,D.; Manolis,A.; Stefanadis,C.; Kallikazaros,I.. Diverse associations of microalbuminuria with C-reactive protein, interleukin-18 and soluble CD 40 ligand in male essential hypertensive subjects. Am J Hypertens. 2006;19:462-466. (Link)

Crist,Scott A.; Sprague,Daniel L.; Ratliff,Timothy L.. Nuclear factor of activated T cells (NFAT) mediates CD154 expression in megakaryocytes. Blood 2008;111:3553-3561. (Link)

Targher,G.; Zoppini,G.. Soluble CD40L in young type 1 diabetic individuals without clinical microvascular and macrovascular complications. Diabetes Care 2004;27:1236-1237. (Link)

Jefferson,A.L.; Massaro,J.M.; Wolf,P.A.; Seshadri,S.; Au,R.; Vasan,R.S.; Larson,M.G.; Meigs,J.B.; Keaney,J.F.,Jr.; Lipinska,I.; Kathiresan,S.; Benjamin,E.J.; DeCarli,C.. Inflammatory biomarkers are associated with total brain volume: The Framingham Heart Study. Neurology 2007;68:1032-1038. (Link)

Ahn,Eugene R.; Lander,Gabriella; Jy,Wenche; Bidot,Carlos J.; Jimenez,Joaquin J.; Horstman,Lawrence L.; Ahn,Yeon S.. Differences of soluble CD40L in sera and plasma: Implications on CD40L assay as a marker of thrombotic risk. Thrombosis Research 2004;114:(Link)

Damas,Jan K.; Otterdal,Kari; Yndestad,Arne; Aass,Halfdan; Solum,Nils O.; Froland,Stig S.; Simonsen,Svein; Aukrust,Pal; Andreassen,Arne K.. Soluble CD40 Ligand in Pulmonary Arterial Hypertension: Possible Pathogenic Role of the Interaction Between Platelets and Endothelial Cells. Circulation 2004;110:999-1005. (Link)

Malarstig,Anders; Lindahl,Bertil; Wallentin,Lars; Siegbahn,Agneta. Soluble CD40L Levels Are Regulated by the -3459 A>G Polymorphism and Predict Myocardial Infarction and the Efficacy of Antithrombotic Treatment in Non-ST Elevation Acute Coronary Syndrome. Arteriosclerosis, Thrombosis, and Vascular Biology 2006;26:1667-1673. (Link)

Halldorsdottir,Anna Margret; Stoker,Joshua; Porche-Sorbet,Rhonda; Eby,Charles S.. Soluble CD40 Ligand Measurement Inaccuracies Attributable to Specimen Type, Processing Time, and ELISA Method. Clinical Chemistry 2005;51:1054-1057. (Link)

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Cosman,D.. A family of ligands for the TNF receptor superfamily. Stem Cells 1994;12:440-455. (Link)

Lane,P.; Brocker,T.; Hubele,S.; Padovan,E.; Lanzavecchia,A.; McConnell,F.. Soluble CD40 ligand can replace the normal T cell-derived CD40 ligand signal to B cells in T cell-dependent activation. J.Exp.Med. 1993;177:1209-1213. (Link)

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