Human sCD40L Instant ELISA

Also known as: TRAP, HIGM 1, T cell antigen Gp39, CD154

CE

SKU# BMS239INSTCE*

Cat. No. Size
BMS239INSTCE 128 tests
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Data for Human sCD40L Instant ELISA.

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  • Data for Human sCD40L Instant ELISA.
Description

Description: The human sCD40L Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of soluble human CD40 ligand. The human sCD40L Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

CD40 is a 48 kD type I glycoprotein and a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.

Details
Reactivity Human
Sample Volume 15 uL
Suitable Sample Types cell culture supernatant, serum, plasma (only high levels)
Sensitivity 0.062 ng/mL
Standard Curve Range 0.16 - 10.0 ng/mL
Components Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sCD40L, HRP-Conjugate (anti-sCD40L monoclonal (murine) antibody) and Sample Diluent, lyophilized
Aluminium pouch(es) with a human sCD40L Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
Reported Applications ELISA
Documentation
TDS Link Download TDS
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References

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Yan,J.C.; Zhu,J.; Gao,L.; Wu,Z.G.; Kong,X.T.; Zong,R.Q.; Zhan,L.Z.. The effect of elevated serum soluble CD40 ligand on the prognostic value in patients with acute coronary syndromes. Clinica Chimica Acta 2004;343:155-159. (Link)

Shea,M.Kyla; Booth,Sarah L.; Massaro,Joseph M.; Jacques,Paul F.; D'Agostino,Ralph B.,Sr.; wson-Hughes,Bess; Ordovas,Jose M.; O'Donnell,Christopher J.; Kathiresan,Sekar; Keaney,John F.,Jr.; Vasan,Ramachandran S.; Benjamin,Emelia J.. Vitamin K and Vitamin D Status: Associations with Inflammatory Markers in the Framingham Offspring Study. American Journal of Epidemiology 2008;167:313-320. (Link)

Saw,Jacqueline; Madsen,Esben Hjorth; Chan,Sammy; Maurer-Spurej,Elisabeth. The ELAPSE (Evaluation of Long-Term Clopidogrel Antiplatelet and Systemic Anti-Inflammatory Effects) Study. Journal of the American College of Cardiology 2008;52:1826-1833. (Link)

YAN,Jin chuan; DING,Shu; LIANG,Yi; MA,Gen shan; ZHU,Jian; FENG,Yi; LUO,Dan. Relationship between upregulation of CD40 system and restenosis in patients after percutaneous coronary intervention4. Acta Pharmacologica Sinica 2007;28:339-343. (Link)

Nassonov,E.L.; Popkova,T.V.; Ilina,A.E.; Klioukvina,N.G.; Panafidina,T.A.; Alexandrova,E.N.; Novikov,A.A.; Novikova,D.S.; Mach,E.S.. ASSOCIATION BETWEEN SOLUBLE CD40 LIGAND AND SUBCLINICAL ATHEROSCLEROSIS IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS. EULAR Meeting Abstracts 2006;65:462-46a. (Link)

Mitani,Hidetsugu; Katayama,Naoyuki; Araki,Hiroto; Ohishi,Kohshi; Kobayashi,Kyoko; Suzuki,Hirohito; Nishii,Kazuhiro; Masuya,Masahiro; Yasukawa,Kiyoshi; Minami,Nobuyuki; Shiku,Hiroshi. Activity of interleukin 6 in the differentiation of monocytes to macrophages and dendritic cells. British Journal of Haematology 2000;109:288-295. (Link)

Takahashi,H.K.; Iwagaki,H.; Mori,S.; Yoshino,T.; Tanaka,N.; Nishibori,M.. á2-Adrenergic receptor agonist induces IL-18 production without IL-12 production. Journal of Neuroimmunology 2004;151:137-147. (Link)

Bleie,O.; Semb,A.G.; Grundt,H.; Nordrehaug,J.E.; Vollset,S.E.; Ueland,P.M.; Nilsen,D.W.T.; Bakken,A.M.; Refsum,H.; Nygard,O.K.. Homocysteine-lowering therapy does not affect inflammatory markers of atherosclerosis in patients with stable coronary artery disease. Journal of Internal Medicine 2007;226:244-253. (Link)

Otterdal,K.; Pedersen,T.M.; Solum,N.O.. Release of soluble CD40 ligand after platelet activation - Studies on the solubilization phase. Thrombosis Research 2004;114:167-177. (Link)

Hammett,Christopher J.K.; Prapavessis,Harry; Baldi,J.Chris; Varo,Nerea; Schoenbeck,Uwe; Ameratunga,Rohan; French,John K.; White,Harvey D.; Stewart,Ralph A.H.. Effects of exercise training on 5 inflammatory markers associated with cardiovascular risk. American Heart Journal 2006;151:(Link)

Sipsas,Nikolaos V.; Sfikakis,Petros P.; Kontos,Athanasios; Kordossis,Theodore. Levels of Soluble CD40 Ligand (CD154) in Serum Are Increased in Human Immunodeficiency Virus Type 1-Infected Patients and Correlate with CD4+ T-Cell Counts. Clinical and Diagnostic Laboratory Immunology 2002;9:558-561. (Link)

Schonbeck,Uwe; Varo,Nerea; Libby,Peter; Buring,Julie; Ridker,Paul M.. Soluble CD40L and Cardiovascular Risk in Women. Circulation 2001;104:2266-2268. (Link)

Varo,Nerea; de Lemos,James A.; Libby,Peter; Morrow,David A.; Murphy,Sabina A.; Nuzzo,Rebecca; Gibson,C.Michael; Cannon,Christopher P.; Braunwald,Eugene; Schonbeck,Uwe. Soluble CD40L: Risk Prediction After Acute Coronary Syndromes459. Circulation 2003;108:1049-1052. (Link)

Jinnin,M.; Ihn,H.; Yazawa,N.; Asano,Y.; Yamane,K.; Tamaki,K.. Circulating soluble CD40 ligand in patients with eosinophilic fasciitis. Annals of the Rheumatic Diseases 2003;62:190-191. (Link)

Solanilla,Anne; Dechanet,Julie; El Andaloussi,Abdel; Dupouy,Moryse; Godard,Francois; Chabrol,Jerome; Charbord,Pierre; Reiffers,Josy; Nurden,Alan T.; Weksler,Babette; Moreau,Jean Francois; Ripoche,Jean. CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells. Blood 2000;95:3758-3764. (Link)

Garlichs,C.D.; Eskafi,S.; Raaz,D.; Schmidt,A.; Ludwig,J.; Herrmann,M.; Klinghammer,L.; Daniel,W.G.; Schmeisser,A.. Patients with acute coronary syndromes express enhanced CD40 ligand/CD154 on platelets. Heart 2001;86:649-655. (Link)

Jinchuan,Y.; Zonggui,W.; Jinming,C.; Li,L.; Xiantao,K.. Upregulation of CD40-CD40 ligand system in patients with diabetes mellitus. Clinica Chimica Acta 2004;339:85-90. (Link)

Czapiga,M.; Kirk,A.D.; Lekstrom-Himes,J.. Platelets deliver costimulatory signals to antigen-presenting cells: A potential bridge between injury and immune... Experimental Hematology 2004;32:135-139. (Link)

Cipollone,Francesco; Mezzetti,Andrea; Porreca,Ettore; Di Febbo,Concetta; Nutini,Michele; Fazia,Maria; Falco,Angela; Cuccurullo,Franco; Davi,Giovanni. Association Between Enhanced Soluble CD40L and Prothrombotic State in Hypercholesterolemia: Effects of Statin Therapy. Circulation 2002;106:399-402. (Link)

Garlichs,C.D.; Kozina,S.; Fateh-Moghadam,S.; Tomandl,B.; Stumpf,C.; Eskafi,S.; Raaz,D.; Schmeisser,A.; Yilmaz,A.; Ludwig,J.; Neundorfer,B.; Daniel,W.G.. Upregulation of CD40-CD40 Ligand (CD154) in Patients With Acute Cerebral Ischemia. Stroke 2003;34:1412-1418. (Link)

Viallard,Jean Francois; Solanilla,Anne; Gauthier,Bruno; Contin,Cecile; Dechanet,Julie; Grosset,Christophe; Moreau,Jean Francois; Praloran,Vincent; Nurden,Paquita; Pellegrin,Jean Luc; Nurden,Alan T.; Ripoche,Jean. Increased soluble and platelet-associated CD40 ligand in essential thrombocythemia and reactive thrombocytosis. Blood 2002;99:2612-2614. (Link)

Aukrust,Pal; Muller,Fredrik; Ueland,Thor; Berget,Trude; Aaser,Elinor; Brunsvig,Anne; Solum,Nils Olav; Forfang,Kolbjorn; Froland,Stig S.; Gullestad,Lars. Enhanced Levels of Soluble and Membrane-Bound CD40 Ligand in Patients With Unstable Angina : Possible Reflection of T Lymphocyte and Platelet Involvement in the Pathogenesis of Acute Coronary Syndromes. Circulation 1999;100:614-620. (Link)

Damas,Jan K.; Jensenius,Mogens; Ueland,Thor; Otterdal,Kari; Yndestad,Arne; Froland,Stig S.; Rolain,Jean Marc; Myrvang,Bjorn; Raoult,Didier; Aukrust,Pal. Increased Levels of Soluble CD40L in African Tick Bite Fever: Possible Involvement of TLRs in the Pathogenic Interaction between Rickettsia africae, Endothelial Cells, and Platelets. The Journal of Immunology 2006;177:2699-2706. (Link)

Hetzel,Jurgen; Balletshofer,Bernd; Rittig,Kilian; Walcher,Daniel; Kratzer,Wolfgang; Hombach,Vinzenz; Haring,Hans Ulrich; Koenig,Wolfgang; Marx,Nikolaus. Rapid Effects of Rosiglitazone Treatment on Endothelial Function and Inflammatory Biomarkers. Arteriosclerosis, Thrombosis, and Vascular Biology 2005;25:1804-1809. (Link)

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Tsubata,T.; Murakami,M.; Nisitani,S.; Honjo,T.. Molecular mechanisms for B lymphocyte selection: induction and regulation of antigen-receptor-mediated apoptosis of mature B cells in normal mice and their defect in autoimmunity-prone mice. Philos.Trans R Soc.Lond B Biol.Sci. 1994;345:297-301. (Link)

Holder,M.J.; Wang,H.; Milner,A.E.; Casamayor,M.; Armitage,R.; Spriggs,M.K.; Fanslow,W.C.; MacLennan,I.C.; Gregory,C.D.; Gordon,J.; .. Suppression of apoptosis in normal and neoplastic human B lymphocytes by CD40 ligand is independent of Bc1-2 induction. Eur.J.Immunol. 1993;23:2368-2371. (Link)

Cosman,D.. A family of ligands for the TNF receptor superfamily. Stem Cells 1994;12:440-455. (Link)

Lane,P.; Brocker,T.; Hubele,S.; Padovan,E.; Lanzavecchia,A.; McConnell,F.. Soluble CD40 ligand can replace the normal T cell-derived CD40 ligand signal to B cells in T cell-dependent activation. J.Exp.Med. 1993;177:1209-1213. (Link)

Brugnoni,D.; Rossi,G.; Tucci,A.; Cattaneo,R.; Airo,P.. Study of CD40 ligand expression in B-cell chronic lymphocytic leukemia. Haematologica 1995;80:440-442. (Link)