Description: This Human Endoglin FlowCytomix Simplex Kit is designed for the measurement of human Endoglin in a bead based immunoassay analyzed on a flow cytometer.
eBioscience bead-based immunoassays follow the same principle as a sandwich immunoassay.
Basic Kits include essential core reagents and are required to successfully perform assays using combinations of Simplex Kits.
Endoglin is a 180 kDa homodimeric co-receptor for members of the TGF-beta superfamily. It is expressed on the surface of endothelial cells, subsets of bone marrow cells (erythroid cell precursors) activated macrophages, fibroblasts, human chondrocytes and smooth muscle cells and profibrogenic liver cells.
A major role for endoglin is in regulating transforming growth factor-beta-dependent vascular remodeling and angiogenesis. Endoglin expression increases during angiogenesis, wound healing, and inflammation, all of which are associated with TGFbeta signaling and alterations in vascular structure. Endoglin binds two isoforms of TGF-beta, TGF-beta 1 and TGF-beta 3 in combination with the signaling complex of TGFá receptors types I and II.
Proteolytic cleavage of the extracellular domain of endoglin gives rise to soluble CD105 (sCD105), which functions to neutralize TGF-beta signaling but enhances signalling pathways involving BMP7 and SMAD1, SMAD5.