Human s90K/Mac-2BP Platinum ELISA

Also known as: Galectin-3-binding protein, Mac-2-binding protein

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS234*

Cat. No. Size
BMS234 96 tests
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Data for Human s90K/Mac-2BP Platinum ELISA.

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  • Data for Human s90K/Mac-2BP Platinum ELISA.
Description

Description: The 90K/Mac-2 BP ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of soluble human 90K/Mac-2 Binding Protein. The 90K/Mac-2 BP ELISA is for research use only. Not for use in diagnostic or therapeutic procedures.

90K/Mac-2 BP is a widely expressed secreted 90 kDa human serum glycoprotein, originally identified in the supernatant of human breast cancer cells. It is identical to Mac-2 BP, the ligand for the secreted lactose-galactose specific S-lectin Mac-2 which is highly expressed by cells of the macrophage-monocyte lineage as well as a variety of other cell types. Although the biological functions and possible roles of 90K/Mac-2 BP are largely unknown, some evidence indicates a role in the immunitary defence mechanism.

Details
Reactivity Human
Sample Volume 100 uL (1:100 prediluted)
Suitable Sample Types cell culture supernatant, serum, plasma
Sensitivity 0.92 ng/mL
Standard Curve Range 12.5 - 200 ng/mL
Components Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human 90K/Mac-2 BP
HRP-Conjugate anti-90K/Mac-2 BP monoclonal (murine) antibody
90K/Mac-2 BP Standard, 200ng/ml; 100ng/ml;50ng/ml;25ng/ml; 12.5ng/ml
Wash Buffer Concentrate 10x (phosphate-buffered saline with Tween 20)
Sample diluent 10x (buffered protein matrix)
Substrate Solution (tetramethyl-benzidine and hydrogen peroxide, stabilized)
Stop Solution (0.3M Sulfuric Acid)
Blue-Dye
Green-Dye
Adhesive Plate Covers
Reagent Labels
Reported Applications ELISA
Documentation
TDS Link Download TDS
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References

Citations: Darcissac,E.C.A.; Vidal,V.; De La Tribonniere,X.; Mouton,Y.; Bahr,G.M.. Variations in serum IL-7 and 90K/Mac-2 binding protein (Mac-2 BP) levels analysed in cohorts of HIV-1 patients and correlated with clinical changes following antiretroviral therapy. Clinical and Experimental Immunology 2001;126:287-294. (Link)

Sardana,Girish; Marshall,John; Diamandis,Eleftherios P.. Discovery of Candidate Tumor Markers for Prostate Cancer via Proteomic Analysis of Cell Culture-Conditioned Medium. Clinical Chemistry 2007;53:429-437. (Link)

References: Glabinski,A.R.; Tuohy,V.K.; Ransohoff,R.M.. Expression of chemokines RANTES, MIP-1alpha and GRO-alpha correlates with inflammation in acute experimental autoimmune encephalomyelitis. Neuroimmunomodulation. 1998;5:166-171. (Link)

Benedetti,Panici P.; Scambia,G.; Baiocchi,G.; Iacobelli,S.; Mancuso,S.. Predictive value of multiple tumor marker assays in second-look procedures for ovarian cancer. Gynecol.Oncol. 1989;35:286-289. (Link)

Iacobelli,S.; Arno,E.; D'Orazio,A.; Coletti,G.. Detection of antigens recognized by a novel monoclonal antibody in tissue and serum from patients with breast cancer. Cancer Res. 1986;46:3005-3010. (Link)

Ullrich,A.; Sures,I.; D'Egidio,M.; Jallal,B.; Powell,T.J.; Herbst,R.; Dreps,A.; Azam,M.; Rubinstein,M.; Natoli,C.; .. The secreted tumor-associated antigen 90K is a potent immune stimulator. J.Biol.Chem. 1994;269:18401-18407. (Link)

Nakayama,M.; Ishidoh,K.; Kojima,Y.; Harada,N.; Kominami,E.; Okumura,K.; Yagita,H.. Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death. J.Immunol. 2003;170:341-348. (Link)


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