Description: This Human PrimeFlow® RNA Assay (3-plex) is an in situ hybridization assay that combines the power of branched-DNA technology with the single cell resolution of flow cytometry to detect up to three RNA targets in a single sample. Branched-DNA technology uses pairs of target-specific probes to achieve up to 8,000-16,000 fold signal amplification from a desired RNA transcript. This assay allows simultaneous detection of RNA targets in combination with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations.
This Human PrimeFlow RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for human ribosomal protein, RPL13A (NM_012423). Target Probe sets for genes of interest are sold separately.
For the study of mouse samples, use the Mouse PrimeFlow RNA Assay (3-plex, cat. 88-18001). For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately.