This site will redirect to thermofisher.com on April 7. Get details >


Human PrimeFlow® RNA Assay (3-plex, see replacement product cat. no. 88-18005)

RUO: For Research Use Only. Not for use in diagnostic procedures.

These products are now available on thermofisher.com. Find out more >

SKU# 88-18009

Cat. No. Size
88-18009-204 40 tests
88-18009-210 100 tests

Data for Human PrimeFlow™ RNA Assay.

Normal human peripheral blood mononuclear cells were unstimulated (left) or stimulated with the Cell...View More

  • Data for Human PrimeFlow™ RNA Assay.
Description

Description: This Human PrimeFlow® RNA Assay (3-plex) is an in situ hybridization assay that combines the power of branched-DNA technology with the single cell resolution of flow cytometry to detect up to three RNA targets in a single sample. Branched-DNA technology uses pairs of target-specific probes to achieve up to 8,000-16,000 fold signal amplification from a desired RNA transcript. This assay allows simultaneous detection of RNA targets in combination with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations.

This Human PrimeFlow RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for human ribosomal protein, RPL13A (NM_012423). Target Probe sets for genes of interest are sold separately.

For the study of mouse samples, use the Mouse PrimeFlow RNA Assay (3-plex, cat. 88-18001). For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately.


Details
Reactivity Human
Components PrimeFlow® RNA Tubes: cat. 19197, store at room temperature
PrimeFlow® RNA Fixation Buffer 1A: cat. 00-18100, store at 2-8°C
PrimeFlow® RNA Fixation Buffer 1B: cat. 00-18200, store at 2-8°C
PrimeFlow® RNA Permeabilization Buffer (10X): cat. 00-18300, store at 2-8°C
PrimeFlow® RNA Fixation Buffer 2 (8X): cat. 00-18400, store at 2-8°C
PrimeFlow® RNA Wash Buffer: cat. 00-19180, store at 2-8°C
PrimeFlow® RNA Target Probe Diluent: cat. 00-19185, store at 2-8°C
PrimeFlow® RNA PreAmp Mix: cat. 00-19187, store at 2-8°C
PrimeFlow® RNA Amp Mix: cat. 00-19190, store at 2-8°C
PrimeFlow® RNA Label Probe Diluent: cat. 00-19183, store at 2-8°C
PrimeFlow® RNA Storage Buffer: cat. 00-19178, store at 2-8°C
Rnase Inhibitor 2 (100X): cat. 00-18501, store at 2-8°C
PrimeFlow® RNA Label Probes: cat. 00-19193, store at -20°C
Rnase Inhibitor 1 (1000X): cat. 00-18500, store at -20°C

Positive Control Probe Sets:
Type 1 Human RPL13A Alexa Fluor® 647 (NM_012423): cat. VA1-13100, store at -20°C
Type 4 Human RPL13A Alexa Fluor® 488 (NM_012423): cat. VA4-13187, store at -20°C
Type 6 Human RPL13A Alexa Fluor® 750 (NM_012423): cat. VA6-13186, store at -20°C
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Related Products
References