Anti-Human Placental Alkaline Phosphatase Purified

Description: The monoclonal antibody 8B6 recognizes human Placental Alkaline Phosphatase (PLAP), a 130 (or 130 with 65 kDa) enzyme expressed by placental syncytiotrophoblastoid cells. This enzyme has slightly different enzyme kinetics from other phosphatases. PLAP can be found anchored to the plasma membrane in addition to the cytoplasm. Expression is found in several germ line cancers; ovarian and testicular. Expression is also present in human embryonic stem cells and bone marrow-derived mesenchymal stromal cells.

This 8B6 antibody reacts with both the Regan and Nagao isozymes.

References: Pilz GA, Ulrich C, Ruh M, Abele H, Schäfer R, Kluba T, Bühring HJ, Rolauffs B, Aicher WK. Human term placenta-derived mesenchymal stromal cells are less prone to osteogenic differentiation than bone marrow-derived mesenchymal stromal cells. Stem Cells Dev. 2011 20:635-46.

Durbin H, Tucker DF, Milligan EM, Bibriw LG, Warne LG, PH, Pookim YL, Bodmer, WF. Production of monoclonal antibodies to placental alkaline phosphatase: Preliminary characterization includes identification of one antibody reactive with routinely fixed histological preparations. Int J Cancer Suppl. 1988 2:50-8. (8B6, mAb characterization)

Goldstein HDL, Blasco L, H Harris Placental alkaline phosphatase in nonmalignant human cervix. Proc Natl Acad Sci U S A. 1980 77(7): 4226–4228.