Human PECAM-1 FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human sPECAM-1 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit PE (cat. BMS8421FF), this kit can be used to detect sPECAM-1 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A5.

PECAM-1 (platelet endothelial cell adhesion molecule-1) also called CD31 and EndoCAM is a newly characterized adhesion molecule that belongs to the immunoglobulin superfamily. PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines.

Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction. The process of leukocyte emigration to the site of inflammation can be dissected into three successive stages: (1) rolling, mediated by the selectins; (2) tight adhesion mediated by ICAMs and their counter-receptors, the integrins; and (3) transmigration of leukocytes through intercellular junctions of vascular endothelial cells which requires PECAM-1. PECAM-1 appears to be able to interact both with itself (homophilic binding) and with other "non-PECAM-1" molecules (heterophilic binding).

PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines.

Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction. PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis. Several data have suggested that PECAM-1 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation.
Besides the membrane-bound form of PECAM-1 a soluble form of the molecule exists, which is 5-10 kDa smaller than cell-associated PECAM-1, and contains the cytoplasmic tail. Soluble PECAM-1 was detected in normal human plasma.

Citations: Wong,C.K.; Ho,A.W.; Tong,P.C.; Yeung,C.Y.; Chan,J.C.; Kong,A.P.; Lam,C.W.. Aberrant expression of soluble co-stimulatory molecules and adhesion molecules in type 2 diabetic patients with nephropathy. J Clin Immunol 2008;28:36-43. (Link)

Michlmayr A.,S.Baumann,M.Zellner,C.Burghuber,P.Schuch,C.Wenzel,R.Bartsch,U.Pluschnig,I.Rech-Weichselbraun,G.Steger,R.Jakesz,M.Gnant and T.Bachleitner-Hofmann,M.Bergmann,R.Oehler. Chemotherapy induces inflammatory response in breast cancer patients. Keystonesymposia, Inflammation, Microenvironment and Cancer 2008;

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