Description: This FlowCytomix Simplex Kit is designed for the measurement of human PDGF-BB in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect PDGF-BB alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
This kit contains bead population B3.
Platelet-derived growth factors (PDGFs) are a family of dimeric disulfide-bound growth factors that bind to two structurally related tyrosine kinase receptors, the PDGF-alpha and PDGF-beta receptors (170 kDa and 180 kDa, respectively). PDGF-AA, PDGF-BB, and PDGF-AB, PDGF-CC and PDGF-DD are PDGFs discovered so far.
The major producers of PDGF are megakaryocytes. alpha-granules of platelets are a major storage site for PDGF, from which it is released after cell activation. PDGF can furthermore be produced by a number of various cell types. PDGF is a potent mitogen and chemotactic factor for mesenchymal cells such as fibroblasts, vascular smooth muscle cells, glomerular mesangial cells, and brain grail cells.
PDGF plays an important role in embryonal development. In kidneys, blood vessels, lungs, connective tissue, and the central nervous system, connective tissue-like cell types depend on PDGF. PDGF affects wound healing; the growth factor increases the rate of repair.