Anti-Human PARP1 Purified

Description: This HC2R8 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa.

The HC2R8 antibody specifically recognizes both the cleaved and non-cleaved forms of PARP.

References: Patel T, Gores GJ, Kaufmann SH. The role of proteases during apoptosis. FASEB J. 1996. Apr;10(5):587-97. Review.

Tewari M, Quan LT, O'Rourke K, Desnoyers S, Zeng Z, Beidler DR, Poirier GG, Salvesen GS, Dixit VM. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell. 1995. Jun 2;81(5):801-9.

Lamarre D, Talbot B, de Murcia G, Laplante C, Leduc Y, Mazen A, Poirier GG. Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study. Biochim Biophys Acta. 1988. Jul 13;950(2):147-60.

Lamarre D, Talbot B, Leduc Y, Muller S, Poirier G. Production and characterization of monoclonal antibodies specific for the functional domains of poly(ADP-ribose) polymerase. Biochem Cell Biol. 1986. Apr;64(4):368-76.

Zahradka P., Ebisuzaki K. Poly(ADP-ribose) polymerase is a zinc metalloenzyme. Eur. J. Biochem. 1984. 142: 503-509.