Anti-Human Naf1 Purified

Description: This 5C4 monoclonal antibody reacts with human Nef-associated factor-1 (Naf-1). This protein is also designated as A20-binding inhibitor of NFkB activation (ABIN-1) and TNFAIP3-interacting protein 1 (TNIP1). Naf-1 is predicted to consist of four leucine zippers and four coiled-coil domains. This protein exists as two isoforms, Naf1α and Naf1β, which are 72-kDa and 68-kDa, respectively, and is expressed in a variety of tissues, with greatest expression in peripheral blood lymphocytes, spleen, and skeletal muscle. Naf1 has also been detected in the Jurkat, Molt-4, and HL60 cell lines.

Naf-1 has been shown to play several biological roles. Naf-1 was originally identified associated with the Nef protein of HIV, and was found to enhance CD4 cell surface expression. Naf-1 has also been demonstrated to inhibit TNF-α- and IL-1-mediated NFkB-dependent gene expression by competing with IKK-γ. Finally, this adaptor protein has been found to associate constitutively with P-selectin glycoprotein ligand 1 (PSGL-1) in human leukocytes. Upon P-selectin binding to PSGL-1, Naf1 is phosphorylated at Tyr552 by Src family kinases, leading to PI3K recruitment and integrin activation.

This antibody recognizes both the unphosphorylated and phosphorylated forms of Naf1α and Naf1β.

References: Wang HB, Wang JT, Zhang L, Geng ZH, Xu WL, Xu T, Huo Y, Zhu X, Plow EF, Chen M, Geng JG. P-selectin primes leukocyte integrin activation during inflammation.Nat Immunol. 2007 Aug;8(8):882-92. (5C4, WB, Pubmed )

Chang MO, Yamamoto N, Horiuchi S, Wu YF, Fujimoto M, Yamamoto N. Production and characterization of a monoclonal antibody specific to Nef-associated factor 1 (Naf1)/A20-binding inhibitor of NF-kappaB activation (ABIN-1). Hybridoma (Larchmt). 2005 Oct;24(5):248-57.

Fukushi M, Dixon J, Kimura T, Tsurutani N, Dixon MJ, Yamamoto N. Identification and cloning of a novel cellular protein Naf1, Nef-associated factor 1, that increases cell surface CD4 expression. FEBS Lett. 1999 Jan 8;442(1):83-8.