Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Description: The polyclonal antibody reacts with human MyD88; the antibody was raised against residues 233-248 of human MyD88 and recognizes human antigens. MyD88, a myeloid differentiation primary response gene, is expressed in a variety of tissues and functions as an adapter molecule in the IL-1 signaling pathway involved in the inflammatory responses induced by cytokines and LPS. MyD88 associates with and recruits IRAK to the IL-1 receptor. Dominant negative mutants of MyD88 attenuate IL-1R-mediated NF-kappaB activation. MyD88 also functions as a regulator molecule for IL-18 receptor and human Toll receptor family. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to LPS. For applications with mouse MyD88, it is recommended that anti-human/mouse MyD88 (C-terminus) (cat. 14-6223) be used.
Applications Reported: This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested: This polyclonal antibody has been tested for immunoblotting (1:500-1:1000 dilution) of MyD88 from Jurkat cell lysate as a positive control. A 35 kDa band can be detected. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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