ZAP-70 (1E7.2) PE

Description: The 1E7.2 antibody reacts with human and mouse ZAP-70, the TCR zeta-associated protein-70. ZAP-70 is a cytosolic protein tyrosine kinase (PTK) and a member of the Syk family of proteins. It is expressed in T and NK cells and is required for TCR signaling and development. ZAP-70 interacts with the TCR complex by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Following activation, ZAP-70 is phosphorylated on several tyrosine residues by two mechanisms; an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck. Tyrosine phosphorylation of ZAP-70 correlates to its increased kinase activity and triggers downstream signaling events.

References: Straus DB, Weiss A. The CD3 chains of the T cell antigen receptor associate with the ZAP-70 tyrosinekinase and are tyrosine phosphorylated after receptor stimulation. J Exp Med. 1993;178:1523-1530.

Chan AC, Iwashima M, Turch CW, Weiss A. ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain. Cell. 1992;71:649-662.