Anti-Human/Mouse Runx1 PE

Description: This RXDMC monoclonal antibody reacts with human and mouse Runx1, which is also known as AML1. This 50-kDa transcription factor is expressed in hematopoietic cells such as myeloid, T, and B cells, but not erythroid cells. Furthermore, Runx1 can be detected in nearly all non-hematopoietic tissues except the brain and heart. Runx1 is involved in several stages of T cell development, including the CD4-CD8- double negative (DN) to CD4+CD8+ double positive (DP) transition and commitment to the CD4 lineage. In addition to its role in thymocyte development, Runx1 has also been implicated in Th2 differentiation and immune homeostasis via direct interaction with Foxp3 in CD4+CD25+ regulatory T cells. Runx1 also affects Th2 differentiation by negatively regulating Gata-3 expression. Finally, the AML1 gene, which encodes Runx1, is a frequent target of translocations in acute myeloid leukemia.

In humans, alternative splicing results in the generation of eleven isoforms. In mice, five isoforms exist as a result of alternative splicing; isoform 4 (aka AML1-C) is the most highly expressed in hematopoietic cells. Sequence analysis indicates that the RXDMC antibody recognizes isoform 4 in mouse and all human isoforms except AML-1FA, AML-1FB, and AML-1FC.

References: Wong WF, Kurokawa M, Satake M, Kohu K. Down-regulation of Runx1 expression by TCR signal involves an autoregulatory mechanism and contributes to IL-2 production. J Biol Chem. 2011 Apr 1;286(13):11110-8.

Collins A, Hewitt SL, Chaumeil J, Sellars M, Micsinai M, Allinne J, Parisi F, Nora EP, Bolland DJ, Corcoran AE, Kluger Y, Bosselut R, Ellmeier W, Chong MM, Littman DR, Skok JA. RUNX transcription factor-mediated association of Cd4 and Cd8 enables coordinate gene regulation. Immunity. 2011 Mar 25;34(3):303-14.

Klunker S, Chong MM, Mantel PY, Palomares O, Bassin C, Ziegler M, Rückert B, Meiler F, Akdis M, Littman DR, Akdis CA. Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. J Exp Med. 2009 Nov 23;206(12):2701-15.

Egawa T, Tillman RE, Naoe Y, Taniuchi I, Littman DR. The role of the Runx transcription factors in thymocyte differentiation and in homeostasis of naive T cells. J Exp Med. 2007 Aug 6;204(8):1945-57.

Okuda T, van Deursen J, Hiebert SW, Grosveld G, Downing JR. AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis. Cell. 1996 Jan 26;84(2):321-30.