Anti-Human/Mouse ROR gamma (t) PE

Also known as: Retinoid-Related Orphan Receptor gamma

Clone: AFKJS-9

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 12-6988

Cat. No. Size
12-6988-82 100 ug
12-6988-80 25 ug
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Data for Anti-Human/Mouse ROR gamma (t) PE.

Surface staining of mouse thymocytes with Anti-Mouse CD4 FITC (cat. 11-0041) and Anti-Mouse CD8a APC...View More

  • Data for Anti-Human/Mouse ROR gamma (t) PE.
Description

Description: The AFKJS-9 monoclonal antibody reacts with the mouse RORγ(t) protein. RORγ is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORα and RORβ. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORγ is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORγt, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ “double-positive” thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

The RORγt isoform differs from RORγ by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the RORγ and RORγt isoforms.

Details
Host Rat
Isotype IgG2a
Reactivity Human, Mouse
Conjugate PE
Laser Blue Laser, Green Laser, Yellow-Green Laser
Excite 488 - 561 nm
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
14-6988 Anti-Human/Mouse ROR gamma (t) Purified IP, WB RUO
17-6988 Anti-Human/Mouse ROR gamma (t) APC 633 - 647 nm 660 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-0041 Anti-Mouse CD4 FITC 488 nm 518 nm FC RUO
17-0081 Anti-Mouse CD8a APC 633 - 647 nm 660 nm FC RUO
00-5523 Foxp3 / Transcription Factor Staining Buffer Set FC GPR
12-4321 Rat IgG2a K Isotype Control PE 488 - 561 nm FC RUO
References

References: Xiao S, Yosef N, Yang J, Wang Y, Zhou L, Zhu C, Wu C, Baloglu E, Schmidt D, Ramesh R, Lobera M, Sundrud MS, Tsai PY, Xiang Z, Wang J, Xu Y, Lin X, Kretschmer K, Rahl PB, Young RA, Zhong Z, Hafler DA, Regev A, Ghosh S, Marson A, Kuchroo VK. Small-Molecule ROR gamma t Antagonists Inhibit T Helper 17 Cell Transcriptional Network by Divergent Mechanisms. Immunity. 2014 Apr 17;40(4):477-89. (AFKJS-9, ChIP, PubMed)

Li L, Kim J, Boussiotis VA.IL-1beta-mediated signals preferentially drive conversion of regulatory T cells but not conventional T cells into IL-17-producing cells. J Immunol. 2010 Oct 1;185(7):4148-53 (AFKJS-9, IC flow, PubMed)

Schmutz S, Bosco N, Chappaz S, Boyman O, Acha-Orbea H, Ceredig R, Rolink AG, Finke D. Cutting edge: IL-7 regulates the peripheral pool of adult RORgamma+ lymphoid tissue inducer cells. J Immunol. 2009 Aug 15;183(4):2217-21 (AFKJS-9, IC flow, PubMed)

Poffenberger MC, Straka N, El Warry N, Fang D, Shanina I, Horwitz MS. Lack of IL-6 during coxsackievirus infection heightens the early immune response resulting in increased severity of chronic autoimmune myocarditis. PLoS One. 2009 Jul 9;4(7):e6207. (AFKJS-9, IC flow, PubMed)

Ivanov II, McKenzie BS, Zhou L, Tadokoro CE, Lepelley A, Lafaille JJ, Cua DJ, Littman DR. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell. 2006 Sep 22;126(6):1121-33.

Sun Z, Unutmaz D, Zou YR, Sunshine MJ, Pierani A, Brenner-Morton S, Mebius RE, Littman DR. Requirement for RORgamma in thymocyte survival and lymphoid organ development. Science. 2000 Jun 30;288(5475):2369-73.

Medvedev A, Yan ZH, Hirose T, Giguère V, Jetten AM. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996 Nov 28; 181(1-2):199-206.


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