Anti-Human/Mouse ROR gamma (t) PE

Description: The AFKJS-9 monoclonal antibody reacts with the mouse RORγ(t) protein. RORγ is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORα and RORβ. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORγ is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORγt, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ “double-positive” thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

The RORγt isoform differs from RORγ by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the RORγ and RORγt isoforms.

References: Li L, Kim J, Boussiotis VA.IL-1beta-mediated signals preferentially drive conversion of regulatory T cells but not conventional T cells into IL-17-producing cells. J Immunol. 2010 Oct 1;185(7):4148-53 (AFKJS-9, IC flow, PubMed)

Schmutz S, Bosco N, Chappaz S, Boyman O, Acha-Orbea H, Ceredig R, Rolink AG, Finke D. Cutting edge: IL-7 regulates the peripheral pool of adult RORgamma+ lymphoid tissue inducer cells. J Immunol. 2009 Aug 15;183(4):2217-21 (AFKJS-9, IC flow, PubMed)

Poffenberger MC, Straka N, El Warry N, Fang D, Shanina I, Horwitz MS. Lack of IL-6 during coxsackievirus infection heightens the early immune response resulting in increased severity of chronic autoimmune myocarditis. PLoS One. 2009 Jul 9;4(7):e6207. (AFKJS-9, ICFC, PubMed)

Ivanov II, McKenzie BS, Zhou L, Tadokoro CE, Lepelley A, Lafaille JJ, Cua DJ, Littman DR. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells.Cell. 2006 Sep 22;126(6):1121-33.

Sun Z, Unutmaz D, Zou YR, Sunshine MJ, Pierani A, Brenner-Morton S, Mebius RE, Littman DR. Requirement for RORgamma in thymocyte survival and lymphoid organ development. Science. 2000 Jun 30;288(5475):2369-73.

He YW, Deftos ML, Ojala EW, Bevan MJ. RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells.Immunity. 1998 Dec;9(6):797-806.

Medvedev A, Yan ZH, Hirose T, Giguère V, Jetten AM. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996 Nov 28; 181(1-2):199-206.