Description: The polyclonal antibody reacts with human, mouse, and rat Toll-interleukin 1 receptor (TIR) domain-containing adapter protein, TIRAP, and was generated using 15 amino acid residues near the C-terminus of mouse TIRAP. TIRAP, a signaling adapter molecule also known as myeloid differentiation protein 88 adapter-like (Mal) is involved in the signaling pathway of TLR4. TIRAP-deficient mice respond normally to the TLR5, TLR7 and TLR9 ligands, as well as to IL-1 and IL-18, but have defects in cytokine production and in activation of the nuclear factor NF-kappaB and mitogen-activated protein kinases in response to lipopolysaccharide, a ligand for TLR4. In addition, TIRAP-deficient mice are also impaired in their responses to ligands for TLR2, TLR1 and TLR6. Immunoblotting with this antibody gives a MW of ~35kDa in human heart lysate.
