Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The monoclonal antibody AP20 recognizes the high molecular weight isoforms of microtubule-associated protein-2 (MAP2a and MAP2b) of human, rat, mouse, bovine, chicken, and xenopus. MAP2 belongs to a family of heat-stable phosphoproteins that are differentially expressed during brain development. MAP2 expression is localized to the cell body and dendrites of neurons and is involved in stabilizing microtubules by crosslinking with intermediate filaments. Expression of MAP2 is commonly used as a neural marker given that its expression becomes more pronounced with neuronal maturation.
This AP20 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval at less than or equal to 5 µg/mL. This AP20 antibody has been tested by immunocytochemistry of methanol-fixed or formalin-fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AC091465.1; DKFZp686I2148; MAP-2; map-2a; map2ab; Microtubule-associated protein 2; OTTHUMP00000163916; OTTHUMP00000208143
Gene Aliases: MAP2
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support