Anti-Foxp3 Alexa Fluor® 488

Also known as: Forkhead Box P3, Scurfin, JM2

Clone: 150D/E4

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-4774

Cat. No.	Size
53-4774-41	25 tests
53-4774-42	100 tests
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Staining of C57BL/6 splenocytes with Anti-Mouse CD25 APC (cat. 17-0251) and Anti-Mouse CD4 PE (cat....View More

Description

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human/primate and mouse/rat Foxp3 protein, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to Foxp3 available from eBioscience, which have been used significantly in scientific literature, include the anti-human Foxp3 PCH101 (cat. 72-5776) and ebio7979 (cat. 12-7979), and the anti-mouse/rat Foxp3 FJK-16s (cat. 72-5775). The 150D/E4 has been mapped to the splice region in Exon 2 found in human cells. The splicing of this region has not been shown to occur in mouse. Characterization of this antibody to the splice variant has been limited to epitope mapping. It should be noted that other antibodies such as PCH101 and 236A/E7 will recognize both the spliced and full length forms of the Foxp3 protein and stain more intensely than 150D/E4 and FJK-16s in human cells.

The 150D/E4 antibody reacts with mouse/rat/human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of FoxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Details

Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
50-4774	Anti-Foxp3 eFluor® 660 (Alexa Fluor® 647 Replacement)	633 - 647 nm	668 nm	FC	RUO
51-4774	Anti-Foxp3 Alexa Fluor® 647 (Discontinued. Refer to Cat. No. 50-4774)	633 - 647 nm	668 nm	FC	RUO
12-4774	Anti-Foxp3 PE	488 - 561 nm	575 nm	FC	RUO
14-4774	Anti-Foxp3 Purified			IHC, IP, WB	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-5521	Foxp3 Fixation/Permeabilization Concentrate and Diluent			FC	GPR
00-5523	Foxp3 / Transcription Factor Staining Buffer Set			FC	GPR
12-0041	Anti-Mouse CD4 PE	488 - 561 nm	575 nm	FC	RUO
17-0251	Anti-Mouse CD25 APC	633 - 647 nm	660 nm	FC	RUO
53-4714	Mouse IgG1 K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Fontenot, JD., Rasmussen, JP., Williams, LM., Dooley, JL., Farr, AG., Rudensky AY. 2005. Regulatory T cell lineage specification by the forkhead transcription factor foxp3. Immunity. 22(3): 329-41.

Hori ,S., Nomura, T., Sakaguchi, S. 2003. Control of regulatory T cell development by the transcription factor Foxp3. Science. 299(5609):1057-61.

Roncador G, Brown PJ, Maestre L, Hue S, Martinez-Torrecuadrada JL, Ling KL, Pratap S, Toms C, Fox BC, Cerundolo V, Powrie F, Banham AH. Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level.Eur J Immunol. 2005 Jun;35(6):1681-91 (150D, IHC, PubMed).

Sakaguchi, S. 2003. The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery. J Clin Invest. 112(9):1310-2.

View More Products

Antigens	Area of Biology	Cell Type	Formats
Foxp3	Adaptive Immunity Metabolism Transcription Factors	T Cells Treg Cells	Alexa Fluor 488