**Starting now: ebioscience.com products are on thermofisher.com. Ebioscience.com to retire in April. Get details >


Anti-Human/Mouse PLZF APC

Also known as: Anti-Mouse promyelocytic leukemia zinc finger protein, zinc finger and BTB domain containing 16, ZBTB16

Clone: 9E12

RUO: For Research Use Only. Not for use in diagnostic procedures.

These products are now available on thermofisher.com. Find out more >

SKU# 17-9322

Cat. No. Size
17-9322-80 25 ug
17-9322-82 100 ug

Data for Anti-Human/Mouse PLZF APC.

LEFT: C57BL/6 thymocytes were surface stained with Anti-Mouse NK1.1 PE-Cyanine7 (cat. 25-5941) and...View More

  • Data for Anti-Human/Mouse PLZF APC.
  • Data for Anti-Human/Mouse PLZF APC.
Description

Description: This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF is expressed by innate lymphoid cells and its progenitor cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintenance of homeostatis. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor.

This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7.

Details
Host Armenian Hamster
Isotype IgG
Reactivity Human, Mouse
Conjugate APC
Laser Red Laser
Emit 660 nm
Excite 633 - 647 nm
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
25-9322 Anti-Human/Mouse PLZF PE-Cyanine7 488 - 561 nm 775 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-5961 Anti-Mouse TCR beta FITC 488 nm 520 nm FC RUO
25-5941 Anti-Mouse NK1.1 PE-Cyanine7 488 - 561 nm 775 nm FC RUO
11-0566 Anti-Human CD56 (NCAM) FITC 488 nm 520 nm FC RUO
17-4888 Armenian Hamster IgG Isotype Control APC 633 - 647 nm 660 nm FC RUO
65-0863 Fixable Viability Dye eFluor® 450 FC GPR
References

References: Lim AW, McKenzie AN. Deciphering the transcriptional switches of innate lymphoid cell programming: the right factors at the right time. Genes Immun. 2015 Apr-May;16(3):177-86.

Constantinides MG, Gudjonson H, McDonald BD, Ishizuka IE, Verhoef PA, Dinner AR, Bendelac A. PLZF expression maps the early stages of ILC1 lineage development. Proc Natl Acad Sci U S A. 2015 Apr 21;112(16):5123-8.

Sadler AJ, Rossello FJ, Yu L, Deane JA, Yuan X, Wang D, Irving AT, Kaparakis-Liaskos M, Gantier MP, Ying H, Yim HC, Hartland EL, Notini AJ, de Boer S, White SJ, Mansell A, Liu JP, Watkins DN, Gerondakis S, Williams BR, Xu D. BTB-ZF transcriptional regulator PLZF modifies chromatin to restrain inflammatory signaling programs. Proc Natl Acad Sci U S A. 2015 Feb 3;112(5):1535-40.

Singh SP, Zhang HH, Tsang H, Gardina PJ, Myers TG, Nagarajan V, Lee CH, Farber JM. PLZF regulates CCR6 and is critical for the acquisition and maintenance of the Th17 phenotype in human cells. J Immunol. 2015 May 1;194(9):4350-61.

Constantinides MG, Bendelac A. Transcriptional regulation of the NKT cell lineage. Curr Opin Immunol. 2013 Apr;25(2):161-7

Alonzo ES, Gottschalk RA, Das J, Egawa T, Hobbs RM, Pandolfi PP, Pereira P, Nichols KE, Koretzky GA, Jordan MS, Sant'Angelo DB. Development of promyelocytic zinc finger and ThPOK-expressing innate gamma delta T cells is controlled by strength of TCR signaling and Id3. J Immunol. 2010 Feb 1;184(3):1268-79.

Kovalovsky D, Alonzo ES, Uche OU, Eidson M, Nichols KE, Sant'Angelo DB. PLZF induces the spontaneous acquisition of memory/effector functions in T cells independently of NKT cell-related signals. J Immunol. 2010 Jun 15;184(12):6746-55.

Lee YJ, Jeon YK, Kang BH, Chung DH, Park CG, Shin HY, Jung KC, Park SH. Generation of PLZF+ CD4+ T cells via MHC class II-dependent thymocyte-thymocyte interaction is a physiological process in humans. J Exp Med. 2010 Jan 18;207(1):237-46.

Savage AK, Constantinides MG, Han J, Picard D, Martin E, Li B, Lantz O, Bendelac A. The transcription factor PLZF directs the effector program of the NKT cell lineage. Immunity. 2008 Sep 19;29(3):391-403.

Martin PJ, Delmotte MH, Formstecher P, Lefebvre P. PLZF is a negative regulator of retinoic acid receptor transcriptional activity. Nucl Recept. 2003 Sep 6;1(1):6


View More Products

AntigensCell TypeFormatsMiscellaneous