Anti-Human/Mouse Notch2 PE

Description: This 16F11 monoclonal antibody reacts with the extracellular domain of human and mouse Notch2, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by γ-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.

Notch2 is expressed on thymocytes (i.e., DN1 and DN2 subsets), activated peripheral T cells, and bone marrow. Moreover, expression of Notch2 has been reported to be altered in various cancers and lymphomas. In addition to its role in stem cell hematopoiesis, Notch2 plays a pivotal role in marginal zone B cell development. Notch2 also interacts with phosphorylated CREB1 to induce granzyme B transcription in cytotoxic T cells.

References: Radtke F, Fasnacht N, Macdonald HR. Notch signaling in the immune system. Immunity. 2010 Jan 29;32(1):14-27.

Fiorini E, Merck E, Wilson A, Ferrero I, Jiang W, Koch U, Auderset F, Laurenti E, Tacchini-Cottier F, Pierres M, Radtke F, Luther SA, Macdonald HR. Dynamic regulation of notch 1 and notch 2 surface expression during T cell development and activation revealed by novel monoclonal antibodies. J Immunol. 2009 Dec 1;183(11):7212-22. (16F11, FC, Pubmed)

Maekawa Y, Minato Y, Ishifune C, Kurihara T, Kitamura A, Kojima H, Yagita H, Sakata-Yanagimoto M, Saito T, Taniuchi I, Chiba S, Sone S, Yasutomo K. Notch2 integrates signaling by the transcription factors RBP-J and CREB1 to promote T cell cytotoxicity. Nat Immunol. 2008 Oct;9(10):1140-7.