Anti-Human/Mouse Notch1 Purified

Description: The Notch family of transmembrane receptors controls cell-fate decisions during the development of many organs in a wide variety of species. After binding its ligand, the Notch receptor is cleaved in its transmembrane domain, and the resulting intracellular domain dissociates from the membrane and translocates to the nucleus, where it is able to suppress the expression of lineage-specific genes by interacting with transcriptional repressors. The mN1A antibody reacts with the intracellular domain of mouse and human Notch1, but not with Notch2, 3, or 4. The mN1A antibody has a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms of Notch1. In the mouse, Notch mRNA is expressed in mouse hematopoietic cells of the fetal liver and adult thymus and bone marrow. In the thymus, Notch1 protein is detected in CD4-CD8- (double-negative) and CD4-CD8+ (single-positive) thymocytes. Studies of Notch1-transgenic cells and Notch1-null mice indicate that the receptor is involved in the regulation of lymphopoiesis and myelopoiesis.

References: Huppert SS, Le A, Schroeter EH, Mumm JS, Saxena MT, Milner LA, Kopan R. Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1.Nature. 2000 Jun 22;405(6789):966-70. Erratum in: Nature 2000 Nov 30;408(6812):616.

Milner LA, Bigas A, Kopan R, Brashem-Stein C, Bernstein ID, Martin DI. Inhibition of granulocytic differentiation by mNotch1.Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13014-9.

Varnum-Finney B, Purton LE, Yu M, Brashem-Stein C, Flowers D, Staats S, Moore KA, Le Roux I, Mann R, Gray G, Artavanis-Tsakonas S, Bernstein ID. The Notch ligand, Jagged-1, influences the development of primitive hematopoietic precursor cells.Blood. 1998 Jun 1;91(11):4084-91.