This site will redirect to thermofisher.com on April 7. Get details >


Anti-Human/Mouse IRF4 PE

Also known as: Interferon regulatory factor 4

Clone: 3E4

RUO: For Research Use Only. Not for use in diagnostic procedures.

These products are now available on thermofisher.com. Find out more >

SKU# 12-9858

Cat. No. Size
12-9858-80 25 ug
12-9858-82 100 ug

Data for Anti-Human/Mouse IRF4 PE.

Normal human peripheral blood cells were either unstimulated (blue historgam) or stimulated for 3...View More

  • Data for Anti-Human/Mouse IRF4 PE.
Description

Description: The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4). IRF4 is a 52 kDa transcription factor with roles in B cell, T cell and macrophage function. In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation. IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1. In T cells, IRF4 has been implicated in regulatory T (Treg), Th2, Th9 and Th17 cell development and function. This transcription factor is upregulated upon T cell activation and is expressed in mature T cells. Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression. IRF4 has also been demonstrated to be involved in macrophage polarization and regulation. Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers.

Details
Host Rat
Isotype IgG1, kappa
Reactivity Human, Mouse
Conjugate PE
Laser Blue Laser, Green Laser, Yellow-Green Laser
Emit 578 nm
Excite 488 - 561 nm
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
11-9858 Anti-Human/Mouse IRF4 FITC 488 nm 520 nm FC RUO
14-9858 Anti-Human/Mouse IRF4 Purified WB RUO
61-9858 Anti-Human/Mouse IRF4 PE-eFluor® 610 488 - 561 nm 607 nm FC RUO
46-9858 Anti-Human/Mouse IRF4 PerCP-eFluor® 710 488 nm 710 nm FC RUO
48-9858 Anti-Human/Mouse IRF4 eFluor® 450 405 nm 445 nm FC RUO
25-9858 Anti-Human/Mouse IRF4 PE-Cyanine7 488 - 561 nm 775 nm FC RUO
50-9858 Anti-Human/Mouse IRF4 eFluor® 660 633 - 647 nm 668 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
65-0865 Fixable Viability Dye eFluor® 780 FC GPR
11-0049 Anti-Human CD4 FITC 488 nm 520 nm FC RUO
16-0037 Anti-Human CD3 Functional Grade Purified FC, FA RUO
00-5523 Foxp3 / Transcription Factor Staining Buffer Set FC GPR
12-4301 Rat IgG1 K Isotype Control PE 488 - 561 nm 578 nm FC RUO
16-0289 Anti-Human CD28 Functional Grade Purified FC, FA RUO
References

References: Cretney E, Xin A, Shi W, Minnich M, Masson F, Miasari M, Belz GT, Smyth GK, Busslinger M, Nutt SL, Kallies A. The transcription factors Blimp-1 and IRF4 jointly control the differentiation and function of effector regulatory T cells. Nat Immunol. 2011 Apr;12(4):304-11.

Satoh T, Takeuchi O, Vandenbon A, Yasuda K, Tanaka Y, Kumagai Y, Miyake T, Matsushita K, Okazaki T, Saitoh T, Honma K, Matsuyama T, Yui K, Tsujimura T, Standley DM, Nakanishi K, Nakai K, Akira S. The Jmjd3-Irf4 axis regulates M2 macrophage polarization and host responses against helminth infection. Nat Immunol. 2010 Oct;11(10):936-44.

Staudt V, Bothur E, Klein M, Lingnau K, Reuter S, Grebe N, Gerlitzki B, Hoffmann M, Ulges A, Taube C, Dehzad N, Becker M, Stassen M, Steinborn A, Lohoff M, Schild H, Schmitt E, Bopp T. Interferon-regulatory factor 4 is essential for the developmental program of T helper 9 cells. Immunity. 2010 Aug; 33(2): 192-202.

Shaffer AL, Emre NC, Romesser PB, Staudt LM. IRF4: Immunity. Malignancy! Therapy? Clin Cancer Res. 2009 May 1;15(9):2954-61

Zheng Y, Chaudhry A, Kas A, deRoos P, Kim JM, Chu TT, Corcoran L, Treuting P, Klein U, Rudensky AY. Regulatory T-cell suppressor program co-opts transcription factor IRF4 to control T(H)2 responses. Nature. 2009 Mar 19;458(7236):351-6. (3E4, IC Flow)

Falini B, Fizzotti M, Pucciarini A, Bigerna B, Marafioti T, Gambacorta M, Pacini R, Alunni C, Natali-Tanci L, Ugolini B, Sebastiani C, Cattoretti G, Pileri S, Dalla-Favera R, Stein H. A monoclonal antibody (MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells, plasma cells, and activated T cells. Blood. 2000 Mar 15;95(6):2084-92.


View More Products

AntigensArea of BiologyFormats