Anti-Human/Mouse High Endothelial Venule Marker Alexa Fluor® 488

Description: This MECA-79 monoclonal antibody recognizes a sulfated carbohydrate epitope present on human and mouse high endothelial venules (HEVs) called peripheral lymph node addressin (PNAd). This glycoprotein is expressed on the luminal surface and in the cytoplasm of HEVs in lymphoid tissues, including peripheral and mesenteric lymph nodes and, to a lesser extent, Peyer’s patches. The MECA-79 antigen has also been detected on follicular B cells in the spleen and appendix in humans, as well as at sites of chronic inflammation. Post-capillary venules or large vessels in the spleen, thymus, or non-lymphoid tissues do not express the MECA-79 antigen and thus cannot be detected with this antibody. This addressin is involved in lymphocyte infiltration and adhesion into secondary lymphoid tissues.

The MECA-79 antibody has been reported to inhibit CD62L (L-selectin)-dependent lymphocyte emigration to HEVs and adhesion in lymph nodes and tonsils.

References: Sinha RK, Yang G, Alexander C, Mage RG. De novo expression of MECA-79 glycoprotein-determinant on developing B lymphocytes in gut-associated lymphoid tissues. Immunology. 2006 Dec;119(4):461-9. (MECA-79, FC, IHC-Fr)

Girard JP, Springer TA. High endothelial venules (HEVs): specialized endothelium for lymphocyte migration. Immunol Today. 1995 Sep;16(9):449-57. Review.

Hemmerich S, Butcher EC, Rosen SD. Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J Exp Med. 1994 Dec 1;180(6):2219-26. (MECA-79, WB)

Streeter PR, Rouse BT, Butcher EC. Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes. J Cell Biol. 1988 Nov;107(5):1853-62. (MECA-79, IHC-Fr, FA)