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Anti-Human/Mouse CD45R (B220) Super Bright 600

Also known as: Ly-5, Lyt-4, T200

Clone: RA3-6B2

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 63-0452

Cat. No. Size
63-0452-80 25 ug
63-0452-82 100 ug

Data for Anti-Human/Mouse CD45R (B220) Super Bright 600.

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3 APC (cat. 17-0032) and 0.25 ug of Rat IgG2a K...View More

  • Data for Anti-Human/Mouse CD45R (B220) Super Bright 600.

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Host Rat
Isotype IgG2a, kappa
Reactivity Human, Mouse
Conjugate Super Bright 600
Laser Violet Laser
Emit 600 nm
Excite 405 nm
Legal Patent pending.
Reported Applications Flow Cytometric Analysis
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
42-0452 Anti-Human/Mouse CD45R (B220) eFluor® 615 595 nm 615 nm ICC, IHC-F RUO
41-0452 Anti-Human/Mouse CD45R (B220) eFluor® 570 555 nm 570 nm ICC, IHC, IHC-F, IHC-P RUO
36-0452 Anti-Human/Mouse CD45R (B220) Functional Grade Biotin FC RUO
45-0452 Anti-Human/Mouse CD45R (B220) PerCP-Cyanine5.5 488 nm 695 nm FC RUO
35-0452 Anti-Human/Mouse CD45R (B220) PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
50-0452 Anti-Human/Mouse CD45R (B220) eFluor® 660 633 - 647 nm 668 nm FC, IHC-F RUO
16-0452 Anti-Human/Mouse CD45R (B220) Functional Grade Purified FC, FA RUO
86-0452 Anti-Human/Mouse CD45R (B220) eVolve™ 655 FC RUO
83-0452 Anti-Human/Mouse CD45R (B220) eVolve™ 605 605 nm FC RUO
47-0452 Anti-Human/Mouse CD45R (B220) APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
48-0452 Anti-Human/Mouse CD45R (B220) eFluor® 450 405 nm 445 nm FC RUO
15-0452 Anti-Human/Mouse CD45R (B220) PE-Cyanine5 488 - 561 nm 667 nm FC RUO
46-0452 Anti-Human/Mouse CD45R (B220) PerCP-eFluor® 710 488 nm 710 nm FC RUO
14-0452 Anti-Human/Mouse CD45R (B220) Purified FC, IHC-F, IHC-P, IP RUO
25-0452 Anti-Human/Mouse CD45R (B220) PE-Cyanine7 488 - 561 nm 775 nm FC RUO
69-0452 Anti-Human/Mouse CD45R (B220) eFluor® 506 405 nm 506 nm FC RUO
12-0452 Anti-Human/Mouse CD45R (B220) PE 488 - 561 nm 578 nm FC RUO
19-0452 Anti-Human/Mouse CD45R (B220) Cyanine5 (Discontinued) 633 - 647 nm 670 nm FC RUO
61-0452 Anti-Human/Mouse CD45R (B220) PE-eFluor® 610 488 - 561 nm 607 nm FC RUO
17-0452 Anti-Human/Mouse CD45R (B220) APC 633 - 647 nm 660 nm FC RUO
56-0452 Anti-Human/Mouse CD45R (B220) Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
53-0452 Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488 488 nm 519 nm FC, IHC-F, IHC-P RUO
13-0452 Anti-Human/Mouse CD45R (B220) Biotin FC, IHC-F, IHC-P RUO
11-0452 Anti-Human/Mouse CD45R (B220) FITC 488 nm 520 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
17-0032 Anti-Mouse CD3 APC 633 - 647 nm 660 nm FC RUO
SB-4400 Super Bright Staining Buffer FC GPR
63-4321 Rat IgG2a K Isotype Control Super Bright 600 405 nm 600 nm FC RUO

References: Schuhmann B, Dietrich A, Sel S, Hahn C, Klingenspor M, Lommatzsch M, Gudermann T, Braun A, Renz H, Nockher WA. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol. 2005 Jun;163(1-2):15-23 (RA3-6B2, IHC frozen, PubMed)

Monteith CE, Chelack BJ, Davis WC, Haines DM. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 1996 Jul;60(3):193-8. (IHC frozen and paraffin, PubMed)

Morse HC 3rd, Davidson WF, Yetter RA, Murphy ED, Roths JB, Coffman RL. Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 1982 Dec;129(6):2612-5.

Coffman, RL. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 1982;69:5-23.

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