Anti-Human/Mouse CD45R (B220) PE-Cy5

Also known as: Ly-5, Lyt-4, T200

Clone: RA3-6B2

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 15-0452

Cat. No.	Size
15-0452-81	50 ug
15-0452-85	500 ug
15-0452-82	100 ug
15-0452-83	200 ug
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Staining of BALB/c splenocytes with 0.06 ug of PE-Cy5 Rat IgG2a Isotype Control (cat. 15-4321) (open...View More

Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Human, Mouse
Conjugate	PE-Cy5
Laser	Blue Laser, Green Laser, Yellow-Green Laser
Emit	670 nm
Excite	488 - 561 nm
Legal	FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 3. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.
Reported Applications	Flow Cytometric Analysis

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
42-0452	Anti-Human/Mouse CD45R (B220) eFluor® 615			ICC, IHC	RUO
35-0452	Anti-Human/Mouse CD45R (B220) PE-Cy5.5	488 - 567 nm	690 nm	FC	RUO
16-0452	Anti-Human/Mouse CD45R (B220) Functional Grade Purified			FC, FA	RUO
13-0452	Anti-Human/Mouse CD45R (B220) Biotin			FC	RUO
58-0452	Anti-Human/Mouse CD45R (B220) Alexa Fluor® 532	532 nm	554 nm	FC	RUO
11-0452	Anti-Human/Mouse CD45R (B220) FITC	488 nm	518 nm	FC	RUO
25-0452	Anti-Human/Mouse CD45R (B220) PE-Cy7	488 - 561 nm	760 nm	FC	RUO
45-0452	Anti-Human/Mouse CD45R (B220) PerCP-Cy5.5	488 nm	690 nm	FC	RUO
48-0452	Anti-Human/Mouse CD45R (B220) eFluor® 450	405 nm	450 nm	FC	RUO
46-0452	Anti-Human/Mouse CD45R (B220) PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
17-0452	Anti-Human/Mouse CD45R (B220) APC	633 - 647 nm	660 nm	FC	RUO
51-0452	Anti-Human/Mouse CD45R (B220) Alexa Fluor® 647 (To Be Discontinued. Refer to Cat. No. 50-0452)	633 nm	668 nm	FC	RUO
14-0452	Anti-Human/Mouse CD45R (B220) Purified			FC, IHC, IP	RUO
IH93-0452	Anti-Human/Mouse CD45R (B220) eFluor® 605NC (for IHC/ICC)	350 - 590 nm	605 nm	ICC, IHC	RUO
95-0452	Anti-Human/Mouse CD45R (B220) eFluor® 650NC	350 - 635 nm	650 nm	FC	RUO
12-0452	Anti-Human/Mouse CD45R (B220) PE	488 - 561 nm	575 nm	FC	RUO
36-0452	Anti-Human/Mouse CD45R (B220) Functional Grade Biotin			FC	RUO
47-0452	Anti-Human/Mouse CD45R (B220) APC-eFluor® 780	633 nm	780 nm	FC	RUO
53-0452	Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488	488 nm	519 nm	FC	RUO
IH95-0452	Anti-Human/Mouse CD45R (B220) eFluor® 650NC (for IHC/ICC)	350 - 635 nm	650 nm	IHC	RUO
50-0452	Anti-Human/Mouse CD45R (B220) eFluor® 660 (Alexa Fluor® 647 Replacement)	633 nm	668 nm	FC	RUO
56-0452	Anti-Human/Mouse CD45R (B220) Alexa Fluor® 700	633 - 647 nm	723 nm	FC	RUO
19-0452	Anti-Human/Mouse CD45R (B220) Cy5	633 - 647 nm	670 nm	FC	RUO
93-0452	Anti-Human/Mouse CD45R (B220) eFluor® 605NC	350 - 590 nm	605 nm	FC	RUO

References

References: Schuhmann B, Dietrich A, et al. 2005. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol. 163(1-2):15-23. (RA3-6B2, IHC frozen, PubMed)

Monteith CE, Chelack BJ, et al. 1996. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 60(3):193-8. (IHC frozen and paraffin, PubMed)

Morse, H. C. d., W. F. Davidson, et al. 1982. Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 129(6): 2612-5.

Coffman, R. L. 1982. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 69: 5-23.

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD45R (B220)	Adaptive Immunity Cell Signaling	B Cells T Cells	PE-Cy5