Anti-Human/Mouse CD45R (B220) APC-eFluor® 780

Also known as: Ly-5, Lyt-4, T200

Clone: RA3-6B2

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 47-0452

Cat. No.	Size
47-0452-80	25 ug
47-0452-82	100 ug
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Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (cat. 11-0031) and 0.25 ug of Rat IgG2a...View More

Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Human, Mouse
Conjugate	APC-eFluor 780
Laser	Red Laser
Emit	780 nm
Excite	633 - 647 nm
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
48-0452	Anti-Human/Mouse CD45R (B220) eFluor® 450	405 nm		FC	RUO
35-0452	Anti-Human/Mouse CD45R (B220) PE-Cyanine5.5	488 - 567 nm	690 nm	FC	RUO
IH93-0452	Anti-Human/Mouse CD45R (B220) eFluor® 605NC (for IHC/ICC)	350 - 590 nm	605 nm	ICC, IHC	RUO
25-0452	Anti-Human/Mouse CD45R (B220) PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
95-0452	Anti-Human/Mouse CD45R (B220) eFluor® 650NC	350 - 635 nm	650 nm	FC	RUO
15-0452	Anti-Human/Mouse CD45R (B220) PE-Cyanine5	488 - 561 nm		FC	RUO
13-0452	Anti-Human/Mouse CD45R (B220) Biotin			FC	RUO
50-0452	Anti-Human/Mouse CD45R (B220) eFluor® 660 (Alexa Fluor® 647 Replacement)	633 - 647 nm	668 nm	FC	RUO
42-0452	Anti-Human/Mouse CD45R (B220) eFluor® 615	595 nm	615 nm	ICC, IHC	RUO
36-0452	Anti-Human/Mouse CD45R (B220) Functional Grade Biotin			FC	RUO
14-0452	Anti-Human/Mouse CD45R (B220) Purified			FC, IHC, IP	RUO
56-0452	Anti-Human/Mouse CD45R (B220) Alexa Fluor® 700	633 - 647 nm	723 nm	FC	RUO
58-0452	Anti-Human/Mouse CD45R (B220) Alexa Fluor® 532	532 nm	561 nm	FC	RUO
93-0452	Anti-Human/Mouse CD45R (B220) eFluor® 605NC	350 - 590 nm	605 nm	FC	RUO
45-0452	Anti-Human/Mouse CD45R (B220) PerCP-Cyanine5.5	488 nm		FC	RUO
17-0452	Anti-Human/Mouse CD45R (B220) APC	633 - 647 nm	660 nm	FC	RUO
53-0452	Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488	488 nm	519 nm	FC	RUO
46-0452	Anti-Human/Mouse CD45R (B220) PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
19-0452	Anti-Human/Mouse CD45R (B220) Cy5	633 - 647 nm	670 nm	FC	RUO
IH95-0452	Anti-Human/Mouse CD45R (B220) eFluor® 650NC (for IHC/ICC)	350 - 635 nm	650 nm	IHC	RUO
11-0452	Anti-Human/Mouse CD45R (B220) FITC	488 nm		FC	RUO
12-0452	Anti-Human/Mouse CD45R (B220) PE	488 - 561 nm		FC	RUO
16-0452	Anti-Human/Mouse CD45R (B220) Functional Grade Purified			FC, FA	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
11-0031	Anti-Mouse CD3e FITC	488 nm		FC	RUO
47-4321	Rat IgG2a K Isotype Control APC-eFluor® 780	633 - 647 nm	780 nm	FC	RUO

References

References: Schuhmann B, Dietrich A, et al. 2005. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol. 163(1-2):15-23. (RA3-6B2, IHC frozen, PubMed)

Monteith CE, Chelack BJ, et al. 1996. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 60(3):193-8. (IHC frozen and paraffin, PubMed)

Morse, H. C. d., W. F. Davidson, et al. 1982. Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 129(6): 2612-5.

Coffman, R. L. 1982. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 69: 5-23.

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD45R (B220)	Adaptive Immunity Cell Signaling	B Cells T Cells	APC-eFluor 780