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Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488

Also known as: Ly-5, Lyt-4, T200

Clone: RA3-6B2

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0452

Cat. No. Size
53-0452-80 25 ug
53-0452-82 100 ug
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Data for Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488.

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg...View More

  • Data for Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488.
Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Details
Host Rat
Isotype IgG2a, kappa
Reactivity Human, Mouse
Conjugate Alexa Fluor 488
Laser Blue Laser
Emit 519 nm
Excite 488 nm
Legal Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
35-0452 Anti-Human/Mouse CD45R (B220) PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
58-0452 Anti-Human/Mouse CD45R (B220) Alexa Fluor® 532 532 nm 561 nm FC RUO
IH93-0452 Anti-Human/Mouse CD45R (B220) eFluor® 605NC (for IHC/ICC) 350 - 590 nm 605 nm ICC, IHC RUO
14-0452 Anti-Human/Mouse CD45R (B220) Purified FC, IHC, IP RUO
36-0452 Anti-Human/Mouse CD45R (B220) Functional Grade Biotin FC RUO
95-0452 Anti-Human/Mouse CD45R (B220) eFluor® 650NC 350 - 635 nm 650 nm FC RUO
16-0452 Anti-Human/Mouse CD45R (B220) Functional Grade Purified FC, FA RUO
19-0452 Anti-Human/Mouse CD45R (B220) Cy5 633 - 647 nm 670 nm FC RUO
11-0452 Anti-Human/Mouse CD45R (B220) FITC 488 nm FC RUO
50-0452 Anti-Human/Mouse CD45R (B220) eFluor® 660 (Alexa Fluor® 647 Replacement) 633 - 647 nm 668 nm FC RUO
13-0452 Anti-Human/Mouse CD45R (B220) Biotin FC RUO
17-0452 Anti-Human/Mouse CD45R (B220) APC 633 - 647 nm 660 nm FC RUO
47-0452 Anti-Human/Mouse CD45R (B220) APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
46-0452 Anti-Human/Mouse CD45R (B220) PerCP-eFluor® 710 488 nm 710 nm FC RUO
56-0452 Anti-Human/Mouse CD45R (B220) Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
42-0452 Anti-Human/Mouse CD45R (B220) eFluor® 615 595 nm 615 nm ICC, IHC RUO
25-0452 Anti-Human/Mouse CD45R (B220) PE-Cyanine7 488 - 561 nm 775 nm FC RUO
45-0452 Anti-Human/Mouse CD45R (B220) PerCP-Cyanine5.5 488 nm FC RUO
48-0452 Anti-Human/Mouse CD45R (B220) eFluor® 450 405 nm FC RUO
15-0452 Anti-Human/Mouse CD45R (B220) PE-Cyanine5 488 - 561 nm FC RUO
IH95-0452 Anti-Human/Mouse CD45R (B220) eFluor® 650NC (for IHC/ICC) 350 - 635 nm 650 nm IHC RUO
12-0452 Anti-Human/Mouse CD45R (B220) PE 488 - 561 nm FC RUO
93-0452 Anti-Human/Mouse CD45R (B220) eFluor® 605NC 350 - 590 nm 605 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
53-4321 Rat IgG2a K Isotype Control Alexa Fluor® 488 488 nm 519 nm FC, ICC, IHC RUO
References

References: Schuhmann B, Dietrich A, et al. 2005. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol. 163(1-2):15-23. (RA3-6B2, IHC frozen, PubMed)

Monteith CE, Chelack BJ, et al. 1996. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 60(3):193-8. (IHC frozen and paraffin, PubMed)

Morse, H. C. d., W. F. Davidson, et al. 1982. Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 129(6): 2612-5.

Coffman, R. L. 1982. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 69: 5-23.

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