Anti-Human/Mouse CD266 (TWEAK Receptor) Purified

Description: The ITEM-2 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducable 14 kDa protein) and cross-reacts with the mouse antigen. Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1 (Cat. No. 16-9915). Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line. ITEM-2 is reported to act as an antagonist in in vitro functional assays.

References: Moreno JA, Muñoz-García B, Martín-Ventura JL, Madrigal-Matute J, Orbe J, Páramo JA, Ortega L, Egido J, Blanco-Colio LM The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis. Atherosclerosis. 2009 Nov;207(1):103-10 (ITEM-2, FA, PubMed)

Nakayama, M., K. Ishidoh, Y. Kojima, N. Harada, E. Kominami, K. Okumura, H. Yagita. Fibroblast Growth Factor-Inducible 14 Mediates Multiple Pathways of TWEAK-Induced Cell Death. J Immunol. 2003, 170: 341-348.

Harada N., M. Nakayama, H. Nakano, Y. Fukuchi, H. Yagita, K. Okumura. Pro-inflammatory Effect of TWEAK/Fn14 Interaction on Human Umbilical Vein Endothelial Cells. Biochem Biophys Res Commun, 2002, 299(3):488-93.

Nakayama M, K. Ishidoh, N. Kayagaki, Y. Kojima, N. Yamaguchi, H. Nakano, E. Kominami, K. Okumura, H. Yagita. Multiple pathways of TWEAK-induced cell death. J Immunol, 2002, 168(2):734-43