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Human/Non-Human Primate Regulatory T Cell Staining Kit #1

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-4999

Cat. No. Size
88-4999-40 Kit

Data for Human/Non-Human Primate Regulatory T Cell Staining Kit #1.

Normal human peripheral blood cells were surface stained with Anti-Human CD4 FITC (left) and...View More

  • Data for Human/Non-Human Primate Regulatory T Cell Staining Kit #1.
Description

Description: This Human/Non-human Primate Regulatory T cell Staining Kit contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human, rhesus, cynomolgus, or chimpanzee peripheral blood cells. This kit may also be used to identify regulatory T cells in baboons, using the CD25 and Foxp3 monoclonal antibodies, however, the monoclonal antibody for CD4 that is included in this kit does not crossreact with baboon.

The OKT4 monoclonal antibody reacts with human, rhesus, cynomolgus, and chimpanzee CD4, a 59 kDa glycoprotein found on the surface of the majority of thymocytes, a subset of mature T cells (T helper cells), and at lower levels on monocytes. The BC96 monoclonal antibody reacts with human, rhesus, cynomolgus, chimpanzee, and baboon CD25 (also known as interleukin-2 receptor alpha, IL-2R alpha), a 55 kDa surface protein expressed by early progenitors of T cells and B cells, by mature, activated T cells and B cells, and at constitutively high levels on regulatory T cells. The PCH101 monoclonal antibody reacts with the amino terminus of human, rhesus, cynomolgus, chimpanzee, and baboon Foxp3, also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Details
Reactivity Human, Monkey
Components Flow Cytometry Staining Buffer (cat. 00-4222): 200 mL, store at 2-8°C.
Fixation/Permeabilization Concentrate (4X) (cat. 00-5123): 30 mL, store at 2-8°C. Avoid agitation.
Fixation/Permeabilization Diluent (cat. 00-5223): 100 mL, store at 2-8°C.
Permeabilization Buffer (10X) (cat. 00-8333): 100 mL, store at 2-8°C. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1x working solution.
Normal Rat Serum (cat. 24-5555): 100 μL, store at 2-8°C.
Anti-Human CD4 FITC (clone OKT4, cat. 11-0048): 25 tests, store at 2-8°C. Light-sensitive material.
Anti-Human CD25 APC (clone BC96, cat. 17-0259): 25 tests, store at 2-8°C. Light-sensitive material.
Anti-Human Foxp3 PE (clone PCH101, cat. 12-4776): 25 tests, store at 2-8°C. Light-sensitive material.
Rat IgG2a K Isotype Control PE (cat. 12-4321): 25 tests, store at 2-8°C. Light-sensitive material.
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Related Products
Cat. No. Name Excite Emit Application Reg.
00-5523 Foxp3 / Transcription Factor Staining Buffer Set FC GPR
17-4714 Mouse IgG1 K Isotype Control APC 633 - 647 nm 660 nm FC RUO
88-8998 Human Regulatory T Cell Staining Kit #2 FC RUO
11-4714 Mouse IgG1 K Isotype Control FITC 488 nm 520 nm FC, ICC, IHC RUO
88-8995 Human Regulatory T Cell Staining Kit #3 FC RUO
88-8999 Human Regulatory T Cell Staining Kit FC RUO
References

References: Seddiki N, Santner-Nanan B, Martinson J, Zaunders J, Sasson S, Landay A, Solomon M, Selby W, Alexander SI, Nanan R, Kelleher A, Fazekas de St Groth B. Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J Exp Med. 2006 Jul 10;203(7):1693-700.

Manigold T, Shin EC, Mizukoshi E, Mihalik K, Murthy KK, Rice CM, Piccirillo CA, Rehermann B. Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees recovered from Hepatitis C. Blood. 2006 Jun 1;107(11):4424-32. (PCH101, crossreactivity to chimpanzee, PubMed)

Ahmadzadeh M, Rosenberg SA. IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients. Blood. 2006 Mar 15;107(6):2409-14.; (PCH101, IC Flow, PubMed)

Hartwig UF, Nonn M, Khan S, Meyer RG, Huber C, Herr W. Depletion of alloreactive T cells via CD69: implications on antiviral, antileukemic and immunoregulatory T lymphocytes. Bone Marrow Transplant. 2006 Feb;37(3):297-305 (PCH101, IC Flow, PubMed)

Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK. Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells. J Immunol. 2005 Dec 15;175(12):8051-9 (PCH101, IC Flow, PubMed)

Lim, H.W., P. Hillsamer, A.H. Banham, and C.H. Kim. Cutting Edge: Direct Suppression of B cells by CD4+CD25+ Regulatory T cells. J Immunol. 2005 Oct 1;175(7):4180-3. (PCH101, IC Flow, PubMed)