Human MIP-1alpha Instant ELISA

Description: The human MIP-1alpha Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of MIP-1alpha levels in cell culture supernatants, serum and plasma. The human MIP-1alpha Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

MIP-1alpha and MIP-1beta belong to the family of cysteine-cysteine (cc) chemokines, RANTES being another prominent member thereof.

Both MIP-1alpha and MIP-1beta are not only chemoattractants but also coactivators of macrophages acting in concert with IFN-gamma as type 1 cytokines.
MIP-1alpha and MIP-1beta are distinct but highly homologous chemokines produced by a variety of host cells in response to various external stimuli and share affinity for their receptor CCR5. The roles of MIP-1alpha and MIP-1beta have been elucidated in response to their affects on cellular and humoral immune response. MIP-1alpha was shown to stimulate strong antigen specific responses, while MIP-1beta promotes antibody responses. Both macrophage inflammatory proteins are however strictly associated with type 1 immune response.

Determination of the expression levels of the MIP-1s turned out to provide important information regarding numerous diseases such as multiple myeloma, allergic asthmatic disorders, acute experimental autoimmune encephalomyelitis, HIV infection, sarcoidosis and sepsis.

Citations: Breitkreutz,I.; Raab,M.; Vallet,S.; Hideshima,T.; Raje,N.; Mitsiades,C.; Chauhan,D.; Okawa,Y.; Munshi,N.; Richardson,P.; Anderson,K.. Lenalidomide inhibits osteoclastogenesis, survival factors and bone-remodeling markers in multiple myeloma. Leukemia 2008;22:1925-1932. (Link)

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Glabinski,A.R.; Tuohy,V.K.; Ransohoff,R.M.. Expression of chemokines RANTES, MIP-1alpha and GRO-alpha correlates with inflammation in acute experimental autoimmune encephalomyelitis. Neuroimmunomodulation. 1998;5:166-171. (Link)

Trumpfheller,C.; Tenner-Racz,K.; Racz,P.; Fleischer,B.; Frosch,S.. Expression of macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES genes in lymph nodes from HIV+ individuals: correlation with a Th1-type cytokine response. Clin.Exp.Immunol. 1998;112:92-99. (Link)

Terpos,E.; Politou,M.; Szydlo,R.; Goldman,J.M.; Apperley,J.F.; Rahemtulla,A.. Serum levels of macrophage inflammatory protein-1 alpha (MIP-1alpha) correlate with the extent of bone disease and survival in patients with multiple myeloma. Br.J.Haematol. 2003;123:106-109. (Link)

Wang,C.R.; Liu,M.F.. Regulation of CCR5 expression and MIP-1alpha production in CD4+ T cells from patients with rheumatoid arthritis. Clin.Exp.Immunol. 2003;132:371-378. (Link)

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Czaplewski,L.G.; McKeating,J.; Craven,C.J.; Higgins,L.D.; Appay,V.; Brown,A.; Dudgeon,T.; Howard,L.A.; Meyers,T.; Owen,J.; Palan,S.R.; Tan,P.; Wilson,G.; Woods,N.R.; Heyworth,C.M.; Lord,B.I.; Brotherton,D.; Christison,R.; Craig,S.; Cribbes,S.; Edwards,R.M.; Evans,S.J.; Gilbert,R.; Morgan,P.; Randle,E.; Schofield,N.; Varley,P.G.; Fisher,J.; Waltho,J.P.; Hunter,M.G.. Identification of amino acid residues critical for aggregation of human CC chemokines macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES. Characterization of active disaggregated chemokine variants. J.Biol.Chem. 1999;274:16077-16084. (Link)

Saito,S.; Kitayama,J.; Jin,Z.X.; Tsuno,N.; Kaisaki,S.; Seto,Y.; Nagawa,H.. Beta-chemokine, macrophage inflammatory protein-1beta (MIP-1beta), is highly expressed in diffuse type human gastric cancers. J.Exp.Clin.Cancer Res. 2003;22:453-459. (Link)

Hashimoto,T.; Abe,M.; Oshima,T.; Shibata,H.; Ozaki,S.; Inoue,D.; Matsumoto,T.. Ability of myeloma cells to secrete macrophage inflammatory protein (MIP)-1alpha and MIP-1beta correlates with lytic bone lesions in patients with multiple myeloma. Br.J.Haematol. 2004;125:38-41. (Link)

Boven,L.A.; Montagne,L.; Nottet,H.S.; De Groot,C.J.. Macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and RANTES mRNA semiquantification and protein expression in active demyelinating multiple sclerosis (MS) lesions 20. Clin.Exp.Immunol. 2000;122:257-263. (Link)

Ashfield,J.T.; Meyers,T.; Lowne,D.; Varley,P.G.; Arnold,J.R.; Tan,P.; Yang,J.C.; Czaplewski,L.G.; Dudgeon,T.; Fisher,J.. Chemical modification of a variant of human MIP-1alpha; implications for dimer structure.. Protein Sci. 2000;9:2047-2053. (Link)

Hub,E.; Rot,A.. Binding of RANTES, MCP-1, MCP-3, and MIP-1alpha to cells in human skin. Am.J.Pathol. 1998;152:749-757. (Link)

Kabashima,H.; Yoneda,M.; Nagata,K.; Hirofuji,T.; Maeda,K.. The presence of chemokine (MCP-1, MIP-1alpha, MIP-1beta, IP-10, RANTES)-positive cells and chemokine receptor (CCR5, CXCR3)-positive cells in inflamed human gingival tissues. Cytokine 2002;20:70-77. (Link)

Cocchi,F.; DeVico,A.L.; Yarchoan,R.; Redfield,R.; Cleghorn,F.; Blattner,W.A.; Garzino-Demo,A.; Colombini-Hatch,S.; Margolis,D.; Gallo,R.C.. Higher macrophage inflammatory protein (MIP)-1alpha and MIP-1beta levels from CD8+ T cells are associated with asymptomatic HIV-1 infection. Proc.Natl.Acad.Sci.U.S.A 2000;97:13812-13817. (Link)

Teruya-Feldstein,J.; Setsuda,J.; Yao,X.; Kingma,D.W.; Straus,S.; Tosato,G.; Jaffe,E.S.. MIP-1alpha expression in tissues from patients with hemophagocytic syndrome. Lab Invest 1999;79:1583-1590. (Link)

Kaburagi,Y.; Shimada,Y.; Nagaoka,T.; Hasegawa,M.; Takehara,K.; Sato,S.. Enhanced production of CC-chemokines (RANTES, MCP-1, MIP-1alpha, MIP-1beta, and eotaxin) in patients with atopic dermatitis. Arch.Dermatol.Res. 2001;293:350-355. (Link)

Kabashima,H.; Yoneda,M.; Nagata,K.; Hirofuji,T.; Ishihara,Y.; Yamashita,M.; Maeda,K.. The presence of chemokine receptor (CCR5, CXCR3, CCR3)-positive cells and chemokine (MCP1, MIP-1alpha, MIP-1beta, IP-10)-positive cells in human periapical granulomas. Cytokine 2001;16:62-66. (Link)

Kim,S.; Jao,S.; Laurence,J.S.; LiWang,P.J.. Structural comparison of monomeric variants of the chemokine MIP-1beta having differing ability to bind the receptor CCR5. Biochemistry 2001;40:10782-10791. (Link)