Anti-Human MICA/B PE

Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind β2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, γδ T cells and αβ CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.

References: Groh V, Steinle A, Bauer S, Spies T. Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells.Science. 1998 Mar 13;279(5357):1737-40. (6D4, FA, FC, PubMed)

Li Z, Groh V, Strong RK, Spies T. A single amino acid substitution causes loss of expression of a MICA allele.Immunogenetics. 2000 Mar;51(3):246-8. (6D4, FC, WB, PubMed)

Jinushi M, Takehara T, Kanto T, Tatsumi T, Groh V, Spies T, Miyagi T, Suzuki T, Sasaki Y, Hayashi N. Critical role of MHC class I-related chain A and B expression on IFN-alpha-stimulated dendritic cells in NK cell activation: impairment in chronic hepatitis C virus infection.J Immunol. 2003 Feb 1;170(3):1249-56. (6D4, FC, FA, PubMed)

Edelmann W, Zervas M, et al. 1996. Neuronal abnormalities in microtubule-associated protein 1B mutant mice. Proc Natl Acad Sci U S A. 93(3):1270-5. (IHC frozen, PubMed)

Hankey KG, Drachenberg CB, et al. 2002. MIC expression in renal and pancreatic allografts. Transplantation. 73(2):304-6. (IHC paraffin, PubMed)

Groh V, Rhinehart R, et al. 1999. Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc Natl Acad Sci U S A. 96(12):6879-84. (IHC frozen and paraffin, PubMed)