Anti-Human MD-2 Purified

Description: The 9B4 antibody reacts with human MD-2, an approximately 30 kDa molecule, which physically associates with the extracellular domain of TLR4. MD-2 is essential for correct intracellular distribution and LPS-recognition of TLR4 since in MD-2(-/-) embryonic fibroblasts, TLR4 was not able to reach the plasma membrane and predominantly resided in the Golgi apparatus, whereas TLR4 was distributed at the leading edge surface of cells in wild-type embryonic fibroblasts. MD-2(-/-) mice do not respond to LPS, do survive endotoxic shock but are susceptible to Salmonella typhimurium infection.

References:
Thompson PA, Tobias PS, Viriyakosol S, Kirkland TN, Kitchens RL. 2003. Lipopolysaccharide (LPS)-binding protein inhibits responses to cell-bound LPS. J Biol Chem. 278(31):28367-71.
Viriyakosol S, Tobias PS, Kitchens RL, Kirkland TN. 2001. MD-2 binds to bacterial lipopolysaccharide. J Biol Chem. 276(41):38044-51.