Anti-Human Mcl-1 Purified

Description: This Ab22 monoclonal antibody reacts with the human myeloid cell leukemia-1 (Mcl-1) protein. This 40 kDa Bcl-2 family member is expressed in B and T cells, macrophages, neutrophils, and hematopoietic stem cells. Mcl-1 is also expressed in non-immune cells such as fibroblasts, epithelial cells, neuroendocrine cells, chondrocytes, and hepatocytes. Reportedly induced by growth factors and cytokines in normal cells, Mcl-1 expression of Mcl-1 can be dysregulated in various leukemias and cancer. Mcl-1 mediates cell survival and proliferation by sequestering the pro-apoptotic Bcl-2 proteins Bak, Bax, and Bim. This protein is also involved in germinal center and memory B cell formation, as well as in myeloid and early lymphoid differentiation. Finally, Mcl-1 activity and dimerization with Bcl-2 family members is dependent on serine and threonine phosphorylation.

References: Vikstrom I, Carotta S, Lüthje K, Peperzak V, Jost PJ, Glaser S, Busslinger M, Bouillet P, Strasser A, Nutt SL, Tarlinton DM. Mcl-1 is Essential for Germinal Center Formation and B Cell Memory. Science. 2010 Oct 7.

Thomas LW, Lam C, Edwards SW. Mcl-1: the molecular regulation of protein function. FEBS Lett. 2010 Jul 16;584(14):2981-9.

Kobayashi S, Lee SH, Meng XW, Mott JL, Bronk SF, Werneburg NW, Craig RW, Kaufmann SH, Gores GJ.Serine 64 phosphorylation enhances the antiapoptotic function of Mcl-1. J Biol Chem. 2007 Jun 22;282(25):18407-17. (Ab22, WB)

Krajewski S, Bodrug S, Krajewska M, Shabaik A, Gascoyne R, Berean K, Reed JC. Immunohistochemical analysis of Mcl-1 protein in human tissues. Differential regulation of Mcl-1 and Bcl-2 protein production suggests a unique role for Mcl-1 in control of programmed cell death in vivo. Am J Pathol. 1995 Jun;146(6):1309-19.