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Anti-Human IL-17AF FITC

Also known as: Interleukin-17AF

Clone: 20LJS09

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 11-9179

Cat. No. Size
11-9179-42 100 tests

Data for Anti-Human IL-17AF FITC.

CD4-enriched human peripheral blood cells were polarized under Th17 conditions (with Human IL-23...View More

  • Data for Anti-Human IL-17AF FITC.
Description

Description: The 20LJS09 monoclonal antibody reacts with human interleukin (IL)-17AF heterodimer. This antibody specifically reacts with this heterodimer and has no reactivity for IL-17A or IL-17F homodimers. IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

Details
Host Mouse
Isotype IgG1, kappa
Reactivity Human
Conjugate FITC
Laser Blue Laser
Emit 520 nm
Excite 488 nm
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
48-9179 Anti-Human IL-17AF eFluor® 450 405 nm 445 nm FC RUO
12-9179 Anti-Human IL-17AF PE 488 - 561 nm 578 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-4975 Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) FC, FA GPR
12-7179 Anti-Human IL-17A PE 488 - 561 nm 578 nm FC RUO
16-0037 Anti-Human CD3 Functional Grade Purified FC, FA RUO
17-0049 Anti-Human CD4 APC 633 - 647 nm 660 nm FC RUO
65-0865 Fixable Viability Dye eFluor® 780 FC GPR
88-8824 Intracellular Fixation & Permeabilization Buffer Set FC GPR
11-4714 Mouse IgG1 K Isotype Control FITC 488 nm 520 nm FC, ICC, IHC RUO
14-8239 Human IL-23 Recombinant Protein ELISA, FA RUO
16-0289 Anti-Human CD28 Functional Grade Purified FC, FA RUO
46-7169 Anti-Human IL-17F PerCP-eFluor® 710 488 nm 710 nm FC RUO
References

References: Chang SH, Dong C. A novel heterodimeric cytokine consisting of IL-17 and IL-17F regulates inflammatory responses. Cell Res. 2007 May;17(5):435-40.

Wright JF, Guo Y, Quazi A, Luxenberg DP, Bennett F, Ross JF, Qiu Y, Whitters MJ, Tomkinson KN, Dunussi-Joannopoulos K, Carreno BM, Collins M, Wolfman NM. Identification of an interleukin 17F/17A heterodimer in activated human CD4+ T cells. J Biol Chem. 2007 May 4;282(18):13447-55.

Liang SC, Tan XY, Luxenberg DP, Karim R, Dunussi-Joannopoulos K, Collins M, Fouser LA. Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides. J Exp Med. 2006 Oct 2;203(10):2271-9.


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