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Anti-Human IL-17AF eFluor® 450

Also known as: Interleukin-17AF

Clone: 20LJS09

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 48-9179

Cat. No. Size
48-9179-42 100 tests

Data for Anti-Human IL-17AF eFluor® 450.

CD4-enriched human peripheral blood cells were polarized under Th17 conditions (with Human IL-23...View More

  • Data for Anti-Human IL-17AF eFluor® 450.
Description

Description: The 20LJS09 monoclonal antibody reacts with human interleukin (IL)-17AF heterodimer. This antibody specifically reacts with this heterodimer and has no reactivity for IL-17A or IL-17F homodimers. IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

Details
Host Mouse
Isotype IgG1, kappa
Reactivity Human
Conjugate eFluor 450
Laser Violet Laser
Emit 445 nm
Excite 405 nm
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
12-9179 Anti-Human IL-17AF PE 488 - 561 nm 578 nm FC RUO
11-9179 Anti-Human IL-17AF FITC 488 nm 520 nm FC RUO
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References

References: Chang SH, Dong C. A novel heterodimeric cytokine consisting of IL-17 and IL-17F regulates inflammatory responses. Cell Res. 2007 May;17(5):435-40.

Wright JF, Guo Y, Quazi A, Luxenberg DP, Bennett F, Ross JF, Qiu Y, Whitters MJ, Tomkinson KN, Dunussi-Joannopoulos K, Carreno BM, Collins M, Wolfman NM. Identification of an interleukin 17F/17A heterodimer in activated human CD4+ T cells. J Biol Chem. 2007 May 4;282(18):13447-55.

Liang SC, Tan XY, Luxenberg DP, Karim R, Dunussi-Joannopoulos K, Collins M, Fouser LA. Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides. J Exp Med. 2006 Oct 2;203(10):2271-9.