Human IL-9 ELISA Ready-SET-Go!®

Description: Interleukin-9 (IL-9) is a proinflammatory cytokine historically believed to be involved in type 2 immune responses. However, recent evidence suggests IL-9 may be secreted by a distinct T Helper lineage called Th9. These cells can either be derived from Th2 cells with TGF-β or differentiated directly from naïve CD4+ T cells with TGF-β and IL-4.

IL-9 is a member of the common cytokine receptor γ chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders.

This human IL-9 ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of human IL-9 protein levels from samples including serum, plasma, and supernatants from cell cultures.The assay demonstrates parallelism in measuring recombinant and native human IL-9 proteins with a standard curve range of 1 pg/ml to 100 pg/ml.

Note: Please use eBioscience Cat. No. 39-8959 as ELISA standard if need additional vial of standard. eBioscience Cat. No. 14-8958 is not suitable for ELISA.

References: Veldhoen M, Uyttenhove C, van Snick J, Helmby H, Westendorf A, Buer J, Martin B, Wilhelm C, Stockinger B. Transforming growth factor-beta 'reprograms' the differentiation of T helper 2 cells and promotes an interleukin 9-producing subset. Nat Immunol. 2008 Dec;9(12):1341-6. Epub 2008 Oct 19.

Matsuzawa S, Sakashita K, Kinoshita T, Ito S, Yamashita T, Koike K. IL-9 enhances the growth of human mast cell progenitors under stimulation with stem cell factor. J Immunol. 2003 Apr 1;170(7):3461-7.

Nicolaides NC, Holroyd KJ, Ewart SL, Eleff SM, Kiser MB, Dragwa CR, Sullivan CD, Grasso L, Zhang LY, Messler CJ, Zhou T, Kleeberger SR, Buetow KH, Levitt RC. Interleukin 9: a candidate gene for asthma. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13175-80.

Kelleher K, Bean K, Clark SC, Leung WY, Yang-Feng TL, Chen JW, Lin PF, Luo W, Yang YC. Human interleukin-9: genomic sequence, chromosomal location, and sequences essential for its expression in human T-cell leukemia virus (HTLV)-I-transformed human T cells. Blood. 1991 Apr 1;77(7):1436-41.